Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 14, Problem 11RQ
Summary Introduction
The majority of the sulfur oxidizing bacteria are autotrophic in nature. Some of the inorganic forms of sulfur such as sulfide and elemental sulfur can be used by chemolithotrophs for their survival, growth, and reproduction. The oxidation of sulfur is generally accompanied with a reduction of nitrate and oxygen.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 14 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 14.1 - Why is it necessary to consider microbial...Ch. 14.1 - What are three reasons that functional traits...Ch. 14.2 - What form of photosynthesis was most likely the...Ch. 14.3 - What are the differentiating properties of the...Ch. 14.3 - Prob. 2MQCh. 14.4 - What is the source of the purple color from which...Ch. 14.4 - Prob. 2MQCh. 14.5 - What are some similarities between purple...Ch. 14.5 - Prob. 2MQCh. 14.6 - Prob. 1MQ
Ch. 14.6 - Prob. 2MQCh. 14.7 - Prob. 1MQCh. 14.7 - Prob. 2MQCh. 14.8 - What types of anoxygenic phototrophs contain...Ch. 14.8 - Prob. 2MQCh. 14.9 - What are the typical electron donors used by...Ch. 14.9 - What bacterial phyla are known to contain...Ch. 14.10 - What are the typical electron donors used by...Ch. 14.10 - Prob. 2MQCh. 14.11 - Describe the energy and carbon metabolism of...Ch. 14.11 - What are some ecological strategies that sulfur...Ch. 14.12 - What mechanisms do free-living diazotraphs use to...Ch. 14.12 - Prob. 2MQCh. 14.13 - Under what conditions would you expect...Ch. 14.13 - Prob. 2MQCh. 14.14 - In what phylogenetic groups are Geobacter and...Ch. 14.14 - Prob. 2MQCh. 14.15 - What habitat characteristics govern the diversity...Ch. 14.15 - How do aerobic neutrophilic iron-oxidizers keep...Ch. 14.16 - Prob. 1MQCh. 14.16 - Prob. 2MQCh. 14.17 - What is the difference between a methanotroph and...Ch. 14.17 - What is unique about the methanotroph...Ch. 14.18 - Prob. 1MQCh. 14.18 - Prob. 2MQCh. 14.19 - Prob. 1MQCh. 14.19 - What are the different ways in which species of...Ch. 14.20 - What are the major differences between spirochetes...Ch. 14.20 - Name two diseases of humans caused by spirochetes.Ch. 14.21 - Prob. 1MQCh. 14.21 - Prob. 2MQCh. 14.22 - Describe how a sheathed bacterium such as...Ch. 14.22 - List two metals that are oxidized by sheathed...Ch. 14.23 - What benefit do magnetic bacteria accrue from...Ch. 14.23 - Would you expect to find greigite or magnetite in...Ch. 14.24 - Prob. 1MQCh. 14.24 - Prob. 2MQCh. 14 - What is convergent evolution and how is it...Ch. 14 - Which bacterial phyla contain phototrophs?Ch. 14 - How are prochlorophytes, such as Prochlorococcus,...Ch. 14 - Compare and contrast the metabolism, morphology,...Ch. 14 - Compare and contrast the metabolism of purple...Ch. 14 - REVIEW QUESTIONS
6. In what group of organisms...Ch. 14 - Prob. 7RQCh. 14 - In what ways is Chloracidobacterium thermophilum...Ch. 14 - Prob. 9RQCh. 14 - In what ways are sulfur-reducing bacteria...Ch. 14 - Prob. 11RQCh. 14 - What are some ways that diazotrophs protect...Ch. 14 - Prob. 13RQCh. 14 - Prob. 14RQCh. 14 - Prob. 15RQCh. 14 - Prob. 16RQCh. 14 - Prob. 17RQCh. 14 - What are the differences between type I and type...Ch. 14 - Prob. 19RQCh. 14 - Compare and contrast the life cycle of Myxococcus...Ch. 14 - Contrast the motility of spirochetes with that of...Ch. 14 - Contrast the life cycle of Hyphomicrobium with...Ch. 14 - Prob. 23RQCh. 14 - In what way does a magnetosome contribute to the...Ch. 14 - Prob. 25RQCh. 14 - Describe a key physiological feature of the...Ch. 14 - APPLICATION QUESTIONS
2. Describe the metabolism...
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- With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forward
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