Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 13, Problem 7P
Interpretation Introduction

(a) Interpretation:

Assuming the solution containing the 5 μ moles of pyruvate dehydrogenase complex and 5 μ moles of all the enzymes of citric acid cycle but none of the metabolic intermediates. This will generate 5 μ moles of CO2 and then reaction stops. The reason for production more CO2 after addition of alcohol dehydrogenase and acetaldehyde should be explained.

Concept introduction:

Pyruvate produced in glycolysis of glucose is oxidised to carbon dioxide and water through a sequence of complicated reaction cycle, popularly named as Krebs cycle. Citric acid and some other tricarboxylic acids occur in the intermediate steps of this reaction sequence, hence the name citric acid cycle and tricarboxylic acid cycle are also given. It is through this cycle of reactions that all food stuff and nutrients are completely oxidised in the cell. All food stuff including carbohydrate must be converted to pyruvate or some other intermediate products for theiroxidation to carbon dioxide and water.

Pictorial representation:

The citric acid cycle with all the reagent and enzymes and metabolic enzymes can be shown as follows:

Biochemistry: Concepts and Connections (2nd Edition), Chapter 13, Problem 7P

Figure: Citric acid cycle

Interpretation Introduction

(b) Interpretation:

The μ moles of acetaldehyde required to allow complete oxidation of the pyruvate to 15 μ moles of CO2 should be calculated.

Concept introduction:

Pyruvate produced in glycolysis of glucose is oxidised to carbon dioxide and water through a sequence of complicated reaction cycle, popularly named as Krebs cycle. Citric acid and some other tricarboxylic acids occur in the intermediate steps of this reaction sequence; hence the name citric acid cycle and tricarboxylic acid cycle are also given. It is through this cycle of reactions that all food stuff and nutrients are completely oxidised in the cell. All food stuff including carbohydrate must be converted to pyruvate or some other intermediate products for theiroxidation to carbon dioxide and water.

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