Concept explainers
Interpretation:
Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained.
Concept Introduction:
The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires
Pictorial representation:
The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-
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Biochemistry: Concepts and Connections (2nd Edition)
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