Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 13, Problem 4P
Interpretation Introduction

Interpretation:

Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained.

Concept Introduction:

The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires Fe2+ ion for its activity and the participation of a sulfhydryl (-SH) group of cysteine or glutothione is essential. Model study with deuterium labelled substrate indicated trans-addition of H and OH groups to the C=C bond of cis-aconitate. The popularly known ferrous wheel mechanism for the transformation was derived from X-ray diffraction study of Fe2+ complexes with these tricarboxylic acids.

Pictorial representation:

The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-

Biochemistry: Concepts and Connections (2nd Edition), Chapter 13, Problem 4P

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