Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Chapter 11, Problem 18P
Summary Introduction
To determine:
The ways by which a prosecutor use DNA from the seed pods to build a case against the defendant.
Introduction:
DNA fingerprinting is defined as the method in which variable elements present in the sequence of DNA are isolated and identified. The DNA is obtained from a sample of cells such as skin, blood, or hair cells. They are a rich source of DNA.
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The following gel shows the results of a crime scene investigation. Lane 1 shows a DNA sample
that was obtained from the scene of a crime (there was evidence of the criminal cutting himself, so
it's the criminal's blood sample that produced the DNA bands in lane 1).
Lanes 2-7 shows a DNA sample from six potential suspects held in connection with the crime.
Arrange the bands labeled A, B, C, D from largest to smallest.
1 2 3 4 5 6 7
OC, A, D, B
A, B, C, D
OB, D, A, C
O D, A, C, B
The Evidence shows which suspect was at the crime scene? ( image of murder investigation)
A) suspect A
B) suspect B
C) suspect C
D) none were at the crime scene
Which of the following choices most likely lists the sizes of the four DNA fragments in lane 6 ( second image) of this agarose gel stained with ethidium bromide? ( Note: the well that the DNA was loaded into is at the top of the gel image).
A) 100 bp, 300 bp, 450 bp, 600 bp.
B) 300 bp, 400 bp, 100 bp, 600 bp
C) 600 bp, 450 bp, 300 bp, 100 bp
D) 400 bp, 300 bp, 600 bp, 100 bp
E) DNA fragment size can't be estimated using an agarose gel.
Examine the gel from a Rape investigation below. If you were the DNA analyst you would conclude that:
DNA size
markers
blood
sexual
assault
evidence
samples
victim
|suspect
A
suspect
B
female
fracțion
male
fraction
1 2 3 4 5 6
A. Suspect B is excluded as the source of the evidence, but Suspect A cannot be excluded.
B. Both Suspects A and B are excluded as the source of the evidence.
C. Suspect A is excluded as the source of the evidence, but Suspect B cannot be excluded.
D. Suspect B cannot be excluded as a source of the evidence. The results with Suspect A are inconclusive.
E. Neither Suspect A or B can be excluded as a source of the evidence.
Chapter 11 Solutions
Genetics: From Genes to Genomes
Ch. 11 - Choose the phrase from the right column that best...Ch. 11 - Would you characterize the pattern of inheritance...Ch. 11 - Would you be more likely to find single nucleotide...Ch. 11 - A recent estimate of the rate of base...Ch. 11 - If you examine Fig. 11.5 closely, you will note...Ch. 11 - Approximately 50 million SNPs have thus far been...Ch. 11 - Mutations at simple sequence repeat SSR loci occur...Ch. 11 - Humans and gorillas last shared a common ancestor...Ch. 11 - In 2015, an international team of scientists...Ch. 11 - Using PCR, you want to amplify an approximately 1...
Ch. 11 - Prob. 11PCh. 11 - The previous problem raises several interesting...Ch. 11 - You want to make a recombinant DNA in which a PCR...Ch. 11 - You sequence a PCR product amplified from a...Ch. 11 - Prob. 15PCh. 11 - The trinucleotide repeat region of the Huntington...Ch. 11 - Sperm samples were taken from two men just...Ch. 11 - Prob. 18PCh. 11 - a. It is possible to perform DNA fingerprinting...Ch. 11 - On July 17, 1918, Tsar Nicholas II; his wife the...Ch. 11 - The figure that follows shows DNA fingerprint...Ch. 11 - Microarrays were used to determine the genotypes...Ch. 11 - A partial sequence of the wild-type HbA allele is...Ch. 11 - a. In Fig. 11.17b, PCR is performed to amplify...Ch. 11 - The following figure shows a partial microarray...Ch. 11 - Scientists were surprised to discover recently...Ch. 11 - The microarray shown in Problem 25 analyzes...Ch. 11 - The figure that follows shows the pedigree of a...Ch. 11 - One of the difficulties faced by human geneticists...Ch. 11 - Now consider a mating between consanguineous...Ch. 11 - The pedigree shown in Fig. 11.22 was crucial to...Ch. 11 - You have identified a SNP marker that in one large...Ch. 11 - The pedigrees indicated here were obtained with...Ch. 11 - Approximately 3 of the population carries a mutant...Ch. 11 - The drug ivacaftor has recently been developed to...Ch. 11 - In the high-throughput DNA sequencing protocol...Ch. 11 - A researcher sequences the whole exome of a...Ch. 11 - As explained in the text, the cause of many...Ch. 11 - Figure 11.26 portrayed the analysis of Miller...Ch. 11 - A research paper published in the summer of 2012...Ch. 11 - Table 11.2 and Fig. 11.27 together portray the...Ch. 11 - The human RefSeq of the entire first exon of a...Ch. 11 - Mutations in the HPRT1 gene in humans result in at...Ch. 11 - Prob. 44P
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- Below is an image of the results of a gel electrophoresis experiment. Lanes 1-4 contain amplified DNA fragments and Lane 5 contains the DNA size marker (DSM). From this image and by only using Lanes 1- 4 which lane matches each description below? a) This lane contains the largest DNA fragment: b) This lane contains the smallest DNA fragment: c) This lane contains a 2.5 kbp DNA fragment: 42 | || ||| | || 12 kb 9.5 kb 6.5 kb 4 kb 2 kb ***arrow_forwardWhich of the following statements regarding DNA fingerprinting is false?a) DNA fingerprinting cannot be used for paternity testingb) DNA profile using STR (short terminal repeats) is unique to an individualc) PCR is used for DNA profilingd) Forensic analysis makes use of SNPs (single nucleotide polymorphisms) in coding sequences to distinguish between individualsarrow_forwardFor what purpose is DNA fingerprinting used A) to sequence DNA from bacteria B) to separate DNA fragments C) to identify individuals who have committed crimes D) to identify single nucleotide polymerasearrow_forward
- Please help me with this questionarrow_forwardSomeone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were left behind, from which DNA was extracted. An individual was caught with all five of the stolen textbooks in his bag. He claims his friend gave them to him. You decide to amplify two regions of the DNA (two loci, plural of locus) and digest the samples, and get the following result on the electrophoresis gel.L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene (Note: assume bands that are close in distance traveled are the same length. Higher concentrations of DNA can make a band appear to travel farther.) Who is most likely the culprit, and how do you know?arrow_forwardSomeone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were left behind, from which DNA was extracted. An individual was caught with all five of the stolen textbooks in his bag. He claims his friend gave them to him. You decide to amplify two regions of the DNA (two loci, plural of locus) and digest the samples, and get the following result on the electrophoresis gel.L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene (Note: assume bands that are close in distance traveled are the same length. Higher concentrations of DNA can make a band appear to travel farther.) Which band patterns are similar?arrow_forward
- How many different loci does the FBI use for DNA profiling (fingerpriniting)? A. 10 B. 12 C. 13 D. 23 E. 22arrow_forwardThey want to determine if the samples contain a toxin or a virus. Material from each sample is used to inoculate leaves on the plant type where the lesions were observed. Both sample 1 and sample 2 produce a lesion. Material from that lesion was then taken and applied to a second set of leaves using either undiluted, or various dilutions of the prepared sample as indicated below for the two different samples. Choose one of the answers below and briefly explain why you choose that answer. A. Both samples contain viruses but they are different viruses. B. Both samples contain toxins but they are different toxins. C. Samples 1 contains a toxin and samples 2 contains a virus. D. Sample 1 contains a virus and sample 2 contains a toxin. No dilution 1/10 1/100 1/1000 Sample 1 Sample 2arrow_forwardThe genus name is single word. Give reason.arrow_forward
- 6751 bp 3652 bp 2827 bp 1568 bp 1118 bp 825 bp 630 bp (-) M CS1 CS2 1 2 3 4 (+) Lane Tube 1 Markers CS 1 CS 2 1 234595 2 6 3 7 4 DNA Standard Marker DNA from crime scene cut with Enzyme 1 DNA from crime scene cut with Enzyme 2 DNA from Suspect 1 cut with Enzyme 1 DNA from Suspect 1 cut with Enzyme 2 DNA from Suspect 2 cut with Enzyme 1 DNA from Suspect 2 cut with Enzyme 2 Using the image here, select all bands (based on approximate sizes) that were present in Suspect #1 cut with Enzyme 2. 600 700 1500 3000 3700arrow_forwardIn your own wordsarrow_forwardSome wildlife conservation organizations claim that bioengineering and cloning can be used to save endangered species. In addition, they suggest that these technologies may be used to bring back extinct species, using DNA from preserved specimens. Discuss the implications of carrying out these procedures.arrow_forward
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