CAMPBELL BIOLOGY MOD MASTERING (18 WEEK)
12th Edition
ISBN: 9780136920335
Author: Urry
Publisher: PEARSON
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Chapter 10, Problem 10.2CR
Summary Introduction
To find: Role of CO2 and H2O in
Introduction: Photosynthesis is the process of converting CO2 and water into simple sugar in the presence of sunlight. Cellular respiration is the process of breaking down simple sugar, obtained from photosynthesis, into CO2 and water and releasing energy in the form of ATP.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 10 Solutions
CAMPBELL BIOLOGY MOD MASTERING (18 WEEK)
Ch. 10.1 - Prob. 1CCCh. 10.1 - Prob. 2CCCh. 10.2 - Prob. 1CCCh. 10.2 - Prob. 2CCCh. 10.2 - Prob. 3CCCh. 10.3 - Prob. 1CCCh. 10.3 - Prob. 2CCCh. 10.3 - Prob. 3CCCh. 10.4 - Prob. 1CCCh. 10.4 - Prob. 2CC
Ch. 10.4 - Prob. 3CCCh. 10.4 - Prob. 4CCCh. 10.4 - Prob. 5CCCh. 10.5 - Prob. 1CCCh. 10.5 - Prob. 2CCCh. 10.5 - Prob. 3CCCh. 10.5 - Prob. 4CCCh. 10.6 - Prob. 1CCCh. 10 - Prob. 10.1CRCh. 10 - Prob. 10.2CRCh. 10 - Prob. 10.3CRCh. 10 - Prob. 10.4CRCh. 10 - Prob. 10.5CRCh. 10 - Prob. 10.6CRCh. 10 - The light reactions of photosynthesis supply the...Ch. 10 - Which of the following sequences correctly...Ch. 10 - How is photosynthesis similar in C4 plants and CAM...Ch. 10 - Prob. 4TYUCh. 10 - Prob. 5TYUCh. 10 - In mechanism, photophosphorylation is most similar...Ch. 10 - Prob. 7TYUCh. 10 - Prob. 8TYUCh. 10 - EVOLUTION CONNECTION Photorespiration can decrease...Ch. 10 - SCIENTIFIC INQUIRY MAKE CONNECTIONS The following...Ch. 10 - Prob. 11TYUCh. 10 - SYNTHESIZE YOUR KNOWLEDGE Watennelon snow in...
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