You’ve made a 25 mM stock of colchicine (a tubulin inhibitor; dissolved in distilled water), which you’d like to use to study the effect of tubulin on phagocytosis in T. pyriformis. Based on published research articles that you’ve read, you’d like to preincubate T. pyriformis with colchicine at a final, effective concentration of 75 µM. Assuming you need 40 µL of the cell+colchicine mixture for your experiment, please explain how you can use your 25 mM solution of colchicine, T. pyriformis cells, and distilled water to create your cell+colchicine mixture
You’ve made a 25 mM stock of colchicine (a tubulin inhibitor; dissolved in distilled water), which you’d like to use to study the effect of tubulin on phagocytosis in T. pyriformis. Based on published research articles that you’ve read, you’d like to preincubate T. pyriformis with colchicine at a final, effective concentration of 75 µM. Assuming you need 40 µL of the cell+colchicine mixture for your experiment, please explain how you can use your 25 mM solution of colchicine, T. pyriformis cells, and distilled water to create your cell+colchicine mixture
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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You’ve made a 25 mM stock of colchicine (a tubulin inhibitor; dissolved in distilled water), which you’d like to use to study the effect of tubulin on phagocytosis in T. pyriformis. Based on published research articles that you’ve read, you’d like to preincubate T. pyriformis with colchicine at a final, effective concentration of 75 µM. Assuming you need 40 µL of the cell+colchicine mixture for your experiment, please explain how you can use your 25 mM solution of colchicine, T. pyriformis cells, and distilled water to create your cell+colchicine mixture.
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