You are working on an enzyme that binds to a steroid hormone. Answer the following questions: • Is this likely to be a globular protein? Explain your answer
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
You are working on an enzyme that binds to a steroid hormone. Answer the following questions: • Is this likely to be a globular protein? Explain your answer. • The substrate is a steroid hormone. How does the enzyme bind to the steroid? (think about the non-covalent interactions that allow this to occur) • Describe the difference in net charge of the surface of this protein compared to the core of the protein. Are they most likely to be different or the same? Explain your answer. • Since this enzyme is extracellular, where in the cell would this enzyme be translated?
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