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Examine this plot. Ligand X binding to Protein Y at different pH 0.9 0.8 pH 7 0.7 0.6 pH 5 0.5 0.4 0.3 0.2 0.1 0.000 0.002 0.004 0.006 0.008 0.010 0012 0.014 Ligand Concentration (mM) Estimate the Kd of ligand X binding to protein Y at pH 7 (correct answer should be within +/- 0.0001 mM) Fraction Binding sites occupied, e

Examine this plot. Ligand X binding to Protein Y at different pH 0.9 0.8 pH 7 0.7 0.6 pH 5 0.5 0.4 0.3 0.2 0.1 0.000 0.002 0.004 0.006 0.008 0.010 0012 0.014 Ligand Concentration (mM) Estimate the Kd of ligand X binding to protein Y at pH 7 (correct answer should be within +/- 0.0001 mM) Fraction Binding sites occupied, e

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Examine this plot. Ligand X binding to Protein Y at different pH 0.9 0.8 pH 7 0.7 0.6 pH 5 0.5 0.4 0.3 0.2 0.1 0.000 0.002 0.004 0.006 0.008 0.010 0.012 0.014 Ligand Concentration (mM) Estimate the Kd of ligand X binding to protein Y at pH 7 (correct answer should be within +/- 0.0001 mM) Fraction Binding sites occupied, e
Transcribed Image Text:Examine this plot. Ligand X binding to Protein Y at different pH 0.9 0.8 pH 7 0.7 0.6 pH 5 0.5 0.4 0.3 0.2 0.1 0.000 0.002 0.004 0.006 0.008 0.010 0.012 0.014 Ligand Concentration (mM) Estimate the Kd of ligand X binding to protein Y at pH 7 (correct answer should be within +/- 0.0001 mM) Fraction Binding sites occupied, e
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