You are studying mutations in a bacterial gene thatcodes for an enzyme whose amino acid sequence isknown. In the wild-type protein, proline is the fifthamino acid from the amino terminal end. In one ofyour mutants with nonfunctional enzyme, you find aserine at position number 5. You subject this mutant tofurther mutagenesis and recover three different strains.Strain A has a proline at position number 5 and actsjust like a wild-type strain. Strain B has tryptophanat position number 5 and also acts like wild type.Strain C has no detectable enzyme function at anytemperature, and you can’t recover any protein thatresembles the enzyme. You mutagenize strain C andrecover a strain (C-1) that has enzyme function. Thesecond mutation in C-1 that is responsible for therecovery of enzyme function does not map at theenzyme locus.a. What is the nucleotide sequence in both strands ofthe wild-type gene at this location?b. Why does strain B have a wild-type phenotype? Whydoes the original mutant with serine at position5 lack function?c. What is the nature of the mutation in strain C?d. What is the second mutation that arose in C-1?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
You are studying mutations in a bacterial gene that
codes for an enzyme whose amino acid sequence is
known. In the wild-type protein, proline is the fifth
amino acid from the amino terminal end. In one of
your mutants with nonfunctional enzyme, you find a
serine at position number 5. You subject this mutant to
further mutagenesis and recover three different strains.
Strain A has a proline at position number 5 and acts
just like a wild-type strain. Strain B has tryptophan
at position number 5 and also acts like wild type.
Strain C has no detectable enzyme function at any
temperature, and you can’t recover any protein that
resembles the enzyme. You mutagenize strain C and
recover a strain (C-1) that has enzyme function. The
second mutation in C-1 that is responsible for the
recovery of enzyme function does not map at the
enzyme locus.
a. What is the
the wild-type gene at this location?
b. Why does strain B have a wild-type
does the original mutant with serine at position
5 lack function?
c. What is the nature of the mutation in strain C?
d. What is the second mutation that arose in C-1?
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