You are a research scientist studying miRNA processing. You currently know everything about thepathway except for one detail: whether Dicer resides in the nucleus or in the cytoplasm. You have anexperiment setup where you have a miRNA that completely complements the GFP (green fluorescentprotein) gene in a model yeast cell. You plan to mutate Exportin 5 and make it dysfunctional, then you willinject synthetic pre-miRNA either in its double strand form or its hairpin single strand form into the cell.Should you inject the synthetic pre-miRNA into the nucleus or the cytoplasm?a)b)If you inject hairpin single stranded pre-miRNA into the appropriate location in the cell, whatcolor do you expect the yeast to be if Dicer is in the nucleus? What color would the yeast be if Dicer is inthe cytoplasm? Briefly explain your reasoning.

Dicer is also known as helicase with RNase motif. It belongs to RNAse III family. Dicer cleaves…

Answered: You are a research scientist studying…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The diagram below shows an imaginary eukaryotic structural gene containing two exons. The exon nucleotides are numbered beginning at the transcription start site and a portion of the intron is not shown to save space: Help Center? transcription start site promoter U STACAGTATAAATGAATTAATTGACGTATGTCAATCGGTAAGT...TCAGGTACT U UUU} Phe UUG} Leu exon 1 3 ATGTCATATTTACTTAATTAACTGCATACAGTTAGCCATTCA...AGTCCATGAATGACTTATGTGCGGTTATTTACTGAT... Second letter C Predict the amino acid sequence of the polypeptide encoded by this structural gene. The genetic code is provided below.
You are studying the effect of various mutations in this gene. The AG sequence (3’splice site) that is within intron 2 [adjacent to Exon 3] has been changed to a CC. What would the resulting mRNA transcript look like? Draw a similar diagram as above and indicate the nucleotide lengths on the mRNA transcript.
A newly identified protein from the cells of the Panopyra plant on Pandora was shown to inhibit translation of its target genes by binding to the 5’ UTR of the mRNA and preventing ribosome binding. A possible way this inhibition may be relieved by an sRNA would be: Group of answer choices a)The sRNA acts as a silencer, suppressing the inhibitory protein and allowing translation to take place. b)The sRNA acts as a decoy, sequestering the inhibitory protein and allowing translation to take place. c)The sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation to take place.
Consider the now dominant variant of the SARS-CoV-2 called the D614G mutation: a) The mutation changes an Aspartate (D, Asp) to a Glycine (G, Gly) at nucleotide position 614 (that’s why it’s called the D614G mutant) in the S1 subunit of the Spike protein. Using only the information above and a codon table, what are the mRNA codon sequences of the 2019-dominant and 2020-dominant Spike proteins? Note the figure above is not needed toanswer the question. Report in 5’ to 3' orientation __________________________________________ b) What type of substitution is this? In your answer, address the following: • The expected substitution in base sequence (e.g., A à C)• If the mutation is synonymous, nonsynonymous, or a frameshift• If the mutation is a transition or a transversion c) The mutation increases infectivity by reducing the stability of the Spike protein such that it can remain in the open conformation more often. The open conformation increases the chances of binding to the host ACE2…
If an antisense RNA is designed to silence the following mRNA sequence, which of the following antisense oligos (a-d) could be used for this purpose? mRNA sequence: 5' UAGGACUAUUAAGGUACACCCAUU 3' O 5' AUCCUGAUAAUUCCAUGUAAAUAA 3' O 5' AAUGGGUGUACCUUAAUAGUCCUA 3' O 5' UAGGACUAUUAAGGUACACCCAUU 3' O 5' UUACCCACAUGGAAUUAUCAGGAU 3¹
MRNAs and eukaryotic cells receive different modifications than those in prokaryotic cells, because eukaryotic mRNAs must be able to accomplish different things. Which of the following describes events that are necessary for an mRNA to be expressed in a eukaryotic cell, but are not necessary for mRNAs in a prokaryotic cell? select all that apply A) introns must be removed from the eukaryotic mRNA B) The mRNA must leave from the nucleus C) transcription factors must bind to the mRNA in a eukaryotic cell after it is transcribed D) A ribsome must bind to the mRNA .
Suppose you had isolated a new transcription factor and wanted to know which genes this protein might regulate. Is there any way that you could use a cDNA microarray of the type shown in the picture to approach this question?
MicroRNAs (miRNAs) ____________________. (select all that apply) Group of answer choices are produced from a precursor miRNA transcript. Are solely derived from double-stranded RNA viruses control gene expression by base-pairing with DNA sequences can degrade RNAs by using their intrinsic catalytic activity
How is the sgRNA different from certain components of the bacterial defense system described?
For each of the following, identify whether that sequence or feature of a typical protein-coding gene would be recognizable in the specified molecule in a typical prokaryotic cell. 5' UTR in DNA? 5' UTR in mRNA? Shine-Dalgarno in DNA? Shine-Dalgarno in polypeptide? Promoter in RNA? Promoter in polypeptide sequence? Stop codon in mRNA? Stop codon in the polypeptide sequence? [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] > <

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