You are a research scientist studying miRNA processing. You currently know everything about the pathway except for one detail: whether Dicer resides in the nucleus or in the cytoplasm. You have an experiment setup where you have a miRNA that completely complements the GFP (green fluorescent protein) gene in a model yeast cell. You plan to mutate Exportin 5 and make it dysfunctional, then you will inject synthetic pre-miRNA either in its double strand form or its hairpin single strand form into the cell. Should you inject the synthetic pre-miRNA into the nucleus or the cytoplasm? a) b) If you inject hairpin single stranded pre-miRNA into the appropriate location in the cell, what color do you expect the yeast to be if Dicer is in the nucleus? What color would the yeast be if Dicer is in the cytoplasm? Briefly explain your reasoning.
You are a research scientist studying miRNA processing. You currently know everything about the pathway except for one detail: whether Dicer resides in the nucleus or in the cytoplasm. You have an experiment setup where you have a miRNA that completely complements the GFP (green fluorescent protein) gene in a model yeast cell. You plan to mutate Exportin 5 and make it dysfunctional, then you will inject synthetic pre-miRNA either in its double strand form or its hairpin single strand form into the cell. Should you inject the synthetic pre-miRNA into the nucleus or the cytoplasm? a) b) If you inject hairpin single stranded pre-miRNA into the appropriate location in the cell, what color do you expect the yeast to be if Dicer is in the nucleus? What color would the yeast be if Dicer is in the cytoplasm? Briefly explain your reasoning.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![You are a research scientist studying miRNA processing. You currently know everything about the
pathway except for one detail: whether Dicer resides in the nucleus or in the cytoplasm. You have an
experiment setup where you have a miRNA that completely complements the GFP (green fluorescent
protein) gene in a model yeast cell. You plan to mutate Exportin 5 and make it dysfunctional, then you will
inject synthetic pre-miRNA either in its double strand form or its hairpin single strand form into the cell.
Should you inject the synthetic pre-miRNA into the nucleus or the cytoplasm?
a)
b)
If you inject hairpin single stranded pre-miRNA into the appropriate location in the cell, what
color do you expect the yeast to be if Dicer is in the nucleus? What color would the yeast be if Dicer is in
the cytoplasm? Briefly explain your reasoning.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Ff5357f27-02dc-4272-8034-c4fad113ceeb%2Ff8ce42e7-1e12-45ff-8aa7-615c0f848704%2F5vlfhz_processed.jpeg&w=3840&q=75)
Transcribed Image Text:You are a research scientist studying miRNA processing. You currently know everything about the
pathway except for one detail: whether Dicer resides in the nucleus or in the cytoplasm. You have an
experiment setup where you have a miRNA that completely complements the GFP (green fluorescent
protein) gene in a model yeast cell. You plan to mutate Exportin 5 and make it dysfunctional, then you will
inject synthetic pre-miRNA either in its double strand form or its hairpin single strand form into the cell.
Should you inject the synthetic pre-miRNA into the nucleus or the cytoplasm?
a)
b)
If you inject hairpin single stranded pre-miRNA into the appropriate location in the cell, what
color do you expect the yeast to be if Dicer is in the nucleus? What color would the yeast be if Dicer is in
the cytoplasm? Briefly explain your reasoning.
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Recommended textbooks for you
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781319114671/9781319114671_smallCoverImage.jpg)
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
![Lehninger Principles of Biochemistry](https://www.bartleby.com/isbn_cover_images/9781464126116/9781464126116_smallCoverImage.gif)
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
![Fundamentals of Biochemistry: Life at the Molecul…](https://www.bartleby.com/isbn_cover_images/9781118918401/9781118918401_smallCoverImage.gif)
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781319114671/9781319114671_smallCoverImage.jpg)
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
![Lehninger Principles of Biochemistry](https://www.bartleby.com/isbn_cover_images/9781464126116/9781464126116_smallCoverImage.gif)
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
![Fundamentals of Biochemistry: Life at the Molecul…](https://www.bartleby.com/isbn_cover_images/9781118918401/9781118918401_smallCoverImage.gif)
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781305961135/9781305961135_smallCoverImage.gif)
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
![Biochemistry](https://www.bartleby.com/isbn_cover_images/9781305577206/9781305577206_smallCoverImage.gif)
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
![Fundamentals of General, Organic, and Biological …](https://www.bartleby.com/isbn_cover_images/9780134015187/9780134015187_smallCoverImage.gif)
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON