1) Which statement below explains the trick in sanger sequencing that produces fluorescently labeled fragments at every length within a fragment?
a) When synthesizing a copy of the DNA to be sequenced, a high concentration of fluorescently labeled dideoxynucleotides (ddNTPs) are used along with a low concentration of deoxynucleotides (dNTPs) to produce the chain termination events at every location in the sequence.
b) When synthesizing a copy of the DNA to be sequenced, fluorescently labeled dideoxynucleotides (ddNTPs) are used instead of deoxynucleotides (dNTPs) to produce the chain termination events at every location in the sequence.
c) When synthesizing a copy of the DNA to be sequenced, a low concentration of fluorescently labeled dideoxynucleotides (ddNTPs) are used along with a high concentration of deoxynucleotides (dNTPs) to produce the chain termination events at every location in the sequence.
d) When synthesizing a copy of the DNA to be sequenced, fluorescently labeled deoxynucleotides (dNTPs) are used instead of dideoxynucleotides (ddNTPs) to produce the chain termination events at every location in the sequence.
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