In the actual experiment, the researchers used 149 sequences to buildtheir sequence logo, which is shown below. There is a stack at eachposition, even if short, because the sequence logo includes moredata. (a) Which three positions in this sequence logo have the mostpredictable bases? Name the most frequent base at each. (b) Whichfour positions have the least predictable bases? How can you tell?

Q: Let’s say that a stretch of repeated AT issuccessfully sequenced. From what you know of the…

A: Sequence assembly involves the alignment and merging of fragments from a DNA (deoxyribonucleic acid)…

Q: A B C D A The following distance matrix: B C D 0.1 0.4 0.3 0.8 0.6 0.2 Using the UPGMA algorithm,…

A: The UPGMA (unweighted pair group method with arithmetic mean) method can be applied to construct a…

Q: You were going to sequence a rice DNA fragment whose sequence was only know at one end, as shown…

A: A primer is a brief sequence of single-stranded DNA that is utilized in the PCR method of polymerase…

Q: A facility says they need 15 μL of a 40 ng/μL solution of plasmid DNA for sequencing. The typical…

A: Appropriate amount of DNA is one the key elements in gene sequencing. Amount of DNA for sequencing…

Q: Your PhD thesis advisor has given you the task of preparing a human genomic DNA library.  3a. How…

A: The length of the human genome is approximately 3.2 billion bases with genes of 20000-25000 protein…

Q: Why did we obtain the sequence from the forward primers separate from the reverse primer?

A: Deoxyribonucleic acid (DNA) is a hereditary molecule that passes genetic information from one…

Q: 5. The A DNA used for digestion was provided at a concentration of 2ug/ul. How much DNA (in…

A: DNA Should be free of contaminants such as phenol, chloroform, alcohol, EDTA, detergents or…

Q: Which one of the following options would be a good way to identify the location of the poly A tail…

A: Pre mRNA undergoes several post-transcriptional modifications. One of these modifications is…

Q: DNA from 100 unrelated individuals from one population of Chinook salmon were amplified at a single…

A: A population is said to be in Hardy Weinberg equilibrium if it satisfies 2 equations; p+q+r=1 and…

Q: Why a multiple sequence alignment is needed for researchers? What inferences can be derived from…

A: When three or more biological sequences are aligned and studied together, it is referred to as…

Q: Why is the lactic acid test done when sepsis is suspected?  Explain why the body produces excess…

A: During sepsis, the serum level of lactate rises above 2 millimoles/Litre.The serum lactate levels…

Q: why is gap opening penalized more than gap extension in doing alignment of sequence similarity?

A: Gap penalties should be use in sequence alignment to obtain the best outcome between the two…

Q: A 13-nucleotide section of an autoradiogram from a Sanger sequencing experiment is depicted in the…

A: The DNA is made up of nucleotides that contain nitrogenous bases, pentose sugar and phosphate group.…

Q: How would you solve this and how would it look? i am confused

A: The objective of this question is to estimate the size of an unknown DNA fragment using a standard…

Q: . In analyzing the number of different bases in a DNAsample, which result would be consistent with…

A: The DNA (Deoxyribonucleic acid) comprises two types of nitrogenous bases including the purines and…

Q: Align two sequences: horizontal - GGAATGG, vertical – ATG, m = 1, mm = 0, g = -1. Use the table…

A: Global Alignment: It is a bioinformatics algorithm used to align protein or nucleotide sequences. A…

Q: You have run a gel with 5 uL of amplified DNA in addition to 1 uL of 6X loading dye. Comparing the…

A: Agarose Gel Electrophoresis (AGE), is an biochemistry technique which is used to estimate DNA…

Q: Method on the following 5 sequences and find the best multiple sequence alignments. Sequence # 1:…

A: Progressive alignment  is a heuristic for multiple sequence alignment that does not optimize any…

Q: How many base substitutions are there between these sequences? Count ONLY the instances where there…

A: Sequence alignment involves the comparison of sequences of a particular gene from various organisms…

Q: Generate a primer pair that is 6 base-pairs long to amplify the inversion, that will notamplify…

A: A primer pair is a set of short, synthetic DNA sequences that are designed to hybridize to opposite…

Q: The xyz gene product, encoded by a new viral strain, has coresponding human homologs. Blast analyses…

A: Scoring matrix  Scoring matrix measures the similarity between two characters in sequence alignment.…

Q: 3a)ClustalX software was used to perform multiple sequence alignment of the following five Nco…

A: Some frequently performed analysis via bioinformatics are DNA sequence analysis, amino acid…

Q: Shown below are several next-generation sequencing reads from a sample you have. Which of the…

A: Genomic research has been revolutionised by next-generation sequencing (NGS), commonly referred to…

Q: The prevalence of highly repetitive sequences seems rather strangeto many geneticists. Do they seem…

A: A tandem repeat is a sequence of two or more DNA base pairs that are repeated in such a manner that…

Q: if i had 3 sequences that were 15 nucleotides long. how would i make an optimal sequence alignment…

A: Aligning one or more sequences by introducing gaps is termed as gap penalty method. The introduction…

Q: are using the restriction enzyme HAEIII to digest different samples of the taster gene isolated from…

A: In gel electrophoresis DNA to be tested is broken down by various restriction endonucleases into…

Q: If you were to isolate genomic DNA from two people and perform restriction enzyme digestion followed…

A: Agarose gel electrophoresis is a technique for distinguishing DNA bands based on size. To…

Q: Looking at each of the fragments (100, 200, 300 base pairs etc.). Why are there so many of the…

A: Restriction fragment length polymorphism (RFLP) is a type of polymorphism that results from…

Q: What part(s) of a nucleotide (namely, phosphate, sugar, and/orbase) is(are) found in the major and…

A: Nucleotides are composed of a phosphate group, a pentose sugar, and a nitrogenous base. The pentose…

Q: What is meant by the term "alignment" in genomic research and in what ways does alignment aid…

A: The study of genomes' design, functioning, development, sequencing, and modification is the emphasis…

Q: Compute the percent identity of the following pairwise sequence alignment: -TGAGACTTAGAGT |..|... |…

A: In bioinformatics, sequence alignment is a process by which two or more sequences are arranged to…

Q: Eukaryotic genomes are replete with repetitive sequences that make genome assembly from…

A: Tandem repeats of the dinucleotide sequence are the repetitive sequences that are found at many…

Q: How would I calculate sanger sequencing fragments that will produce green

A: Sanger sequencing is a method used to determine the sequence of DNA by incorporating…

Q: Using the Dynamic Programming algorithm for pairwise local alignment we covered in class, construct…

A:

Q: cule is subjected to complete striction digestion by (1) OKI alone, (2) HindIII alone, and (3) both…

A: Restriction enzymes are a special class of enzyme that cleaves the DNA molecule in particular sites.…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.