In the actual experiment, the researchers used 149 sequences to buildtheir sequence logo, which is shown below. There is a stack at eachposition, even if short, because the sequence logo includes moredata. (a) Which three positions in this sequence logo have the mostpredictable bases? Name the most frequent base at each. (b) Whichfour positions have the least predictable bases? How can you tell?

Q: Let’s say that a stretch of repeated AT issuccessfully sequenced. From what you know of the…

A: Sequence assembly involves the alignment and merging of fragments from a DNA (deoxyribonucleic acid)…

Q: A B C D A The following distance matrix: B C D 0.1 0.4 0.3 0.8 0.6 0.2 Using the UPGMA algorithm,…

A: The UPGMA (unweighted pair group method with arithmetic mean) method can be applied to construct a…

Q: one should run the following

A: Pairwise sequence alignment is one form of a sequence alignment technique, where we compare only two…

Q: 1. Look at the cut DNA sequences shown below in i) and ii). a) What restriction enzyme(s) was/were…

A: To help you better understand the answers above, here are non-technical explanations of each…

Q: Why is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?

A: Frederick Sanger and his colleagues created Sanger Sequencing, often known as 'chain termination…

Q: What is the role of Mg2+ in genomic DNA extraction?

A: DNA extraction refers to the method of isolating and purifying DNA from a cell. It involves…

Q: Your PhD thesis advisor has given you the task of preparing a human genomic DNA library.  3a. How…

A: The length of the human genome is approximately 3.2 billion bases with genes of 20000-25000 protein…

Q: Why did we obtain the sequence from the forward primers separate from the reverse primer?

A: Deoxyribonucleic acid (DNA) is a hereditary molecule that passes genetic information from one…

Q: a BLASTP

A: Introduction  The Basic Local Alignment Search Tool (BLAST) observes districts of nearby similitude…

Q: 5. The A DNA used for digestion was provided at a concentration of 2ug/ul. How much DNA (in…

A: DNA Should be free of contaminants such as phenol, chloroform, alcohol, EDTA, detergents or…

Q: Which one of the following options would be a good way to identify the location of the poly A tail…

A: Pre mRNA undergoes several post-transcriptional modifications. One of these modifications is…

Q: DNA from 100 unrelated individuals from one population of Chinook salmon were amplified at a single…

A: A population is said to be in Hardy Weinberg equilibrium if it satisfies 2 equations; p+q+r=1 and…

Q: Why a multiple sequence alignment is needed for researchers? What inferences can be derived from…

A: When three or more biological sequences are aligned and studied together, it is referred to as…

Q: why is gap opening penalized more than gap extension in doing alignment of sequence similarity?

A: Gap penalties should be use in sequence alignment to obtain the best outcome between the two…

Q: Although DNA transposons are abundant in the genomes of multicellular eukaryotes, class 1 elements…

A: The transposable elements are sequences which keeps on changing their position in the human genome,…

Q: . In analyzing the number of different bases in a DNAsample, which result would be consistent with…

A: The DNA (Deoxyribonucleic acid) comprises two types of nitrogenous bases including the purines and…

Q: Align two sequences: horizontal - GGAATGG, vertical – ATG, m = 1, mm = 0, g = -1. Use the table…

A: Global Alignment: It is a bioinformatics algorithm used to align protein or nucleotide sequences. A…

Q: Method on the following 5 sequences and find the best multiple sequence alignments. Sequence # 1:…

A: Progressive alignment  is a heuristic for multiple sequence alignment that does not optimize any…

Q: How many base substitutions are there between these sequences? Count ONLY the instances where there…

A: Sequence alignment involves the comparison of sequences of a particular gene from various organisms…

Q: Generate a primer pair that is 6 base-pairs long to amplify the inversion, that will notamplify…

A: A primer pair is a set of short, synthetic DNA sequences that are designed to hybridize to opposite…

Q: The xyz gene product, encoded by a new viral strain, has coresponding human homologs. Blast analyses…

A: Scoring matrix  Scoring matrix measures the similarity between two characters in sequence alignment.…

Q: 3a)ClustalX software was used to perform multiple sequence alignment of the following five Nco…

A: Some frequently performed analysis via bioinformatics are DNA sequence analysis, amino acid…

Q: The prevalence of highly repetitive sequences seems rather strangeto many geneticists. Do they seem…

A: A tandem repeat is a sequence of two or more DNA base pairs that are repeated in such a manner that…

Q: if i had 3 sequences that were 15 nucleotides long. how would i make an optimal sequence alignment…

A: Aligning one or more sequences by introducing gaps is termed as gap penalty method. The introduction…

Q: are using the restriction enzyme HAEIII to digest different samples of the taster gene isolated from…

A: In gel electrophoresis DNA to be tested is broken down by various restriction endonucleases into…

Q: You wish to align a 300 bp nucleotide sequence to a genome. Which of the following pairwise sequence…

A: Pairwise sequence alignment is a fundamental process in bioinformatics that includes aligning two…

Q: In hybridizing microarrays, we use a solution that contains {3X SSC, 0.5% SDS, 5ug/ml salmon sperm…

A: The ratio of mass of solute present in a solution relative to the volume of the solution represents…

Q: If you were to isolate genomic DNA from two people and perform restriction enzyme digestion followed…

A: Agarose gel electrophoresis is a technique for distinguishing DNA bands based on size. To…

Q: Looking at each of the fragments (100, 200, 300 base pairs etc.). Why are there so many of the…

A: Restriction fragment length polymorphism (RFLP) is a type of polymorphism that results from…

Q: What is meant by the term "alignment" in genomic research and in what ways does alignment aid…

A: The study of genomes' design, functioning, development, sequencing, and modification is the emphasis…

Q: Eukaryotic genomes are replete with repetitive sequences that make genome assembly from…

A: Tandem repeats of the dinucleotide sequence are the repetitive sequences that are found at many…

Q: Using the Dynamic Programming algorithm for pairwise local alignment we covered in class, construct…

A:

Q: What is a DNA library? Do you think this name is appropriate?

A: In terms of molecular biology, a library can be defined as the collection of DNA fragments that are…

Q: The optimal design of primers is critical to the effective amplification of DNA sequences. i)…

A: INTRODUCTION Polymerase chain reaction (PCR) is a method widely wont to rapidly make millions to…

Q: Assume 2x108 reads of 75 bps long are obtained from a next-generation sequencing experiment to…

A: Next-generation sequencing relies on the continuous sequencing of the genome parts and later…

Q: Extracting DNA From Strawberries What is the purpose of adding the extraction buffer (soap + salt)?…

A: Strawberries are octoploid that means they have 8 copies of each chromosome in the cell.…

Q: Southern and Northern blotting are powerful molecular biological tools based on hybridization of…

A: There are basically 4 types of blotting: Southern blotting. Western blotting. Northern blotting.…

Q: Once the steps for RNA seq have been identified, write the steps in the order in which they are…

A: The word Transcriptome refers to the amount of total RNA transcripts in an organism. Transcriptome…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.