When the enzyme is incubated with iodoacetate, it is observed that glyceraldehyde-3- phosphate still binds to the modified enzyme, but it is not oxidized. What conclusion can be drawn from this experiment?

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter20: Electron Transport And Oxidative Phosphorylation
Section: Chapter Questions
Problem 12P
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The glyceraldehyde-3-phosphate-deshydrogenase
glyceraldehyde-3-phosphate in 1,3-bisphosphoglycerate.
catalyses the
catalyses
-CH₂ -CHOH—CHO + Pi + NAD*
the oxidation of
CH₂ CHOH-COQ + H* + NADH
When the enzyme is incubated with iodoacetate, it is observed that glyceraldehyde-3-
phosphate still binds to the modified enzyme, but it is not oxidized. What conclusion
can be drawn from this experiment?
Data: iodoacetate ICH₂COO is an alkylating reagent that react with free sulfhydryl
groups (-SH) to form (-S-CH₂COO).
Transcribed Image Text:The glyceraldehyde-3-phosphate-deshydrogenase glyceraldehyde-3-phosphate in 1,3-bisphosphoglycerate. catalyses the catalyses -CH₂ -CHOH—CHO + Pi + NAD* the oxidation of CH₂ CHOH-COQ + H* + NADH When the enzyme is incubated with iodoacetate, it is observed that glyceraldehyde-3- phosphate still binds to the modified enzyme, but it is not oxidized. What conclusion can be drawn from this experiment? Data: iodoacetate ICH₂COO is an alkylating reagent that react with free sulfhydryl groups (-SH) to form (-S-CH₂COO).
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