The glyceraldehyde-3-phosphate-deshydrogenaseglyceraldehyde-3-phosphate in 1,3-bisphosphoglycerate.catalyses thecatalyses-CH₂ -CHOH—CHO + Pi + NAD*the oxidation ofCH₂ CHOH-COQ + H* + NADHWhen the enzyme is incubated with iodoacetate, it is observed that glyceraldehyde-3-phosphate still binds to the modified enzyme, but it is not oxidized. What conclusioncan be drawn from this experiment?Data: iodoacetate ICH₂COO is an alkylating reagent that react with free sulfhydrylgroups (-SH) to form (-S-CH₂COO).

Enzymes are made up of amino acids, and the sequence as well as the arrangement of those amino acids…

Answered: When the enzyme is incubated with…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The enzyme pyruvate carboxylase is discovered in a bacterium that was thought not to contain it; in this case study, you’ll see how researchers study and characterize the enzyme, and, ultimately, show how it fits into a metabolic pathway. Following purification of the PYC enzyme by avidin-Sepharose affinity chromatography, the investigators carried out several experiments to characterize the enzyme. First, they ran samples of the enzyme on denaturing and non-denaturing gels; the results are shown in the figure to the right. In addition, they ran the protein through a calibrated gel filtration column, the results of which indicated that the PYC enzyme had a molecular weight of 540 kiloDaltons. In the figure, the rightmost column are where molecular weight standards of various sizes would occur if they’d been on the gels. The catalytic properties of the PYC enzyme were assessed following purification. The activity of the enzyme was assayed in the presence of ATP, pyruvate, bicarbonate…
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When the enzyme is incubated with iodoacetate, it is observed that glyceraldehyde-3- phosphate still binds to the modified enzyme, but it is not oxidized. What conclusion can be drawn from this experiment?