(This is taken mainly from Lehninger Principles of Biochemistry, 6th ed., ch. 6, #12, p. 239.) The kinetic data below are for the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase ("COX"), producing prostaglandin G2, the precursor of other prostaglandins and of thromboxanes. Velocity was measured as a function of the concentration of the substrate arachidonic acid in the absence and in the presence of 10 mg/ml ibuprofen (C13H1802). The molecular mass of the undissociated acid form of ibuprofen is 206 g/mol. (Structure of the anionic form is on p. 846 of Lehninger Principles of Biochemistry, 6th ed.) The reciprocals of velocity and of [substrate] have been calculated for you! [arachidonic acid] (mm) 0.5 1.0 1.5 2.5 3.5 1/[S] (mM)-1 2.00 1.00 0.667 0.400 0.286 I velocity (rate of formation of PGG2) (mm/min) 23.5 32.2 36.9 41.8 44.0 1/v (mm/min)-1 0.0426 0.0311 0.0271 0.0239 0.0227 Velocity with 10 mg/ml ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 1/v with ibuprofen (mm/min-1 0.0600 0.0396 0.0328 0.0270 0.0257 Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine Km and Vmax in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units for all numerical answers.
(This is taken mainly from Lehninger Principles of Biochemistry, 6th ed., ch. 6, #12, p. 239.) The kinetic data below are for the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase ("COX"), producing prostaglandin G2, the precursor of other prostaglandins and of thromboxanes. Velocity was measured as a function of the concentration of the substrate arachidonic acid in the absence and in the presence of 10 mg/ml ibuprofen (C13H1802). The molecular mass of the undissociated acid form of ibuprofen is 206 g/mol. (Structure of the anionic form is on p. 846 of Lehninger Principles of Biochemistry, 6th ed.) The reciprocals of velocity and of [substrate] have been calculated for you! [arachidonic acid] (mm) 0.5 1.0 1.5 2.5 3.5 1/[S] (mM)-1 2.00 1.00 0.667 0.400 0.286 I velocity (rate of formation of PGG2) (mm/min) 23.5 32.2 36.9 41.8 44.0 1/v (mm/min)-1 0.0426 0.0311 0.0271 0.0239 0.0227 Velocity with 10 mg/ml ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 1/v with ibuprofen (mm/min-1 0.0600 0.0396 0.0328 0.0270 0.0257 Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine Km and Vmax in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units for all numerical answers.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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