How does one plot the lineweaver burk plot using this data. ## Kinetic Data Analysis of Cyclooxygenase Reaction### IntroductionThis experiment analyzes the kinetics of the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase (COX). This enzyme is critical in producing prostaglandin G2, a precursor to various other prostaglandins and thromboxanes. The reaction's velocity was studied based on varying concentrations of substrate arachidonic acid, both in the absence and presence of ibuprofen.### Key Details- **Ibuprofen Concentration**: 10 mg/ml- **Molecular Mass of Undissociated Ibuprofen**: 206 g/mol### Data Presented| [Arachidonic Acid] (mM) | 1/[S] (mM^-1) | Velocity (Rate of Formation of PGG₂) (mM/min) | 1/v (mM/min^-1) | Velocity with 10 mg/ml Ibuprofen (mM/min) | 1/v with Ibuprofen (mM/min^-1) ||-------------------------|---------------|---------------------------------------------|-----------------|------------------------------------------|----------------------------------|| 0.5 | 2.00 | 23.5 | 0.0426 | 16.67 | 0.0600 || 1.0 | 1.00 | 32.2 | 0.0311 | 25.25 | 0.0396 || 1.5 | 0.667 | 36.9 | 0.0271 | 30.49 | 0.0328 || 2.0 | 0.500 | 41.8 | 0.0239 | 37.04 | 0.0270 || 3.5 | 0.286 | 44.0 | 0.0227 | 38.91 | 0.0257 |### Experimental TaskUse the provided data to create a Lineweaver-Burk plot (double reciprocal plot). From this graph, determine the kinetic parameters \( K_m \) and \( V_{max} \) for the enzyme activity, both in the presence and absence of ibuprofen.### Instructions- Plot \( 1/[S] \) on

A Lineweaver-Burk plot(double reciprocal plot) is used to calculate the Km and Vmax. The…

velocity (rate of formation Velocity with 10 mg/ml ibuprofen (mM/min) [arachidonic acid] (mM) 1/[S] 1/v 1/v (mM) (mM/min with ibuprofen (mM/min of PGG,) (mM/min) 23.5 0.5 1.0 1.5 2.5 3.5 16.67 25.25 30.49 37.04 38.91 2.00 0.0426 0.0311 0.0271 0.0239 0.0227 0,0600 0.0396 1.00 0.667 0.400 0.286 32.2 36.9 41.8 44.0 0.0328 0.0270 0.0257 Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine K, and V in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units for all numerical answers.

velocity (rate of formation Velocity with 10 mg/ml ibuprofen (mM/min) [arachidonic acid] (mM) 1/[S] 1/v 1/v (mM) (mM/min with ibuprofen (mM/min of PGG,) (mM/min) 23.5 0.5 1.0 1.5 2.5 3.5 16.67 25.25 30.49 37.04 38.91 2.00 0.0426 0.0311 0.0271 0.0239 0.0227 0,0600 0.0396 1.00 0.667 0.400 0.286 32.2 36.9 41.8 44.0 0.0328 0.0270 0.0257 Use a Lineweaver-Burk plot (double reciprocal plot) on the graph paper below to determine K, and V in the absence and in the presence of ibuprofen. Show your work/reasoning, and state units for all numerical answers.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

How does one plot the lineweaver burk plot using this data.

## Kinetic Data Analysis of Cyclooxygenase Reaction

### Introduction
This experiment analyzes the kinetics of the cyclooxygenase reaction catalyzed by prostaglandin H2 synthase (COX). This enzyme is critical in producing prostaglandin G2, a precursor to various other prostaglandins and thromboxanes. The reaction's velocity was studied based on varying concentrations of substrate arachidonic acid, both in the absence and presence of ibuprofen.

### Key Details
- **Ibuprofen Concentration**: 10 mg/ml
- **Molecular Mass of Undissociated Ibuprofen**: 206 g/mol

### Data Presented

| [Arachidonic Acid] (mM) | 1/[S] (mM^-1) | Velocity (Rate of Formation of PGG₂) (mM/min) | 1/v (mM/min^-1) | Velocity with 10 mg/ml Ibuprofen (mM/min) | 1/v with Ibuprofen (mM/min^-1) |
|-------------------------|---------------|---------------------------------------------|-----------------|------------------------------------------|----------------------------------|
| 0.5 | 2.00 | 23.5 | 0.0426 | 16.67 | 0.0600 |
| 1.0 | 1.00 | 32.2 | 0.0311 | 25.25 | 0.0396 |
| 1.5 | 0.667 | 36.9 | 0.0271 | 30.49 | 0.0328 |
| 2.0 | 0.500 | 41.8 | 0.0239 | 37.04 | 0.0270 |
| 3.5 | 0.286 | 44.0 | 0.0227 | 38.91 | 0.0257 |

### Experimental Task
Use the provided data to create a Lineweaver-Burk plot (double reciprocal plot). From this graph, determine the kinetic parameters \( K_m \) and \( V_{max} \) for the enzyme activity, both in the presence and absence of ibuprofen.

### Instructions
- Plot \( 1/[S] \) on

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