When researchers obtain genomic sequence data from organisms with little known genetic information, they often search foropen reading frames (ORFS) to classify potential genes. Suppose scientists have collected the sequence5'-TAATGCCTAGTACCGGACTGAGTCAGTGTCTA–3'3'-ATTACGGATCATGGCCTGACTCAGTCACAGAT-5'Select the reading frame that corresponds to each translational product. You may want to use the Codons table and the AminoAcid Abbreviations table. Stop codons are noted as a dash (-).MPSTGLSQCLNA-YRTESVS-TLTQSGTRH-CLVPD-VSV Amino Acid Abbreviations3-letterabbreviation abbreviation3-letterabbreviation abbreviationName1-letterName1-letterAlanineAlaAMethionineMetMCysteineCysAspAsparagineProlineAsnAspartic acidGlutamic acidPhenylalanineGlycineProPGluGlutamineGlnPheFArginineArgRGlyGSerineSerThreonineValineHistidineHisHThrIsoleucineIleIleIValVLysineLysKTryptophanTrpWLeucineLeuLTyrosineTyrYcode Amino Acid code Amino Acid code Amino Acid code Amino AcidU UUUPheUCUSerUAUTyrUGUCysUUCUCCUACUGCUUALeuUCAUAASTOPUGASTOPAUUGUGUAGSTOPUGGTrpC CUULeuCUProCAUHisCGUArgCUCCCCÁCCGCCỦACCACAAGlnCGACUGCCGCAGCGGA AUUIleACUThrAAUAsnAGUSerAUCACCAACAGCAUAACAAAALysAGAArgAUGMetACGAAGAGGG GUUValGCUAlaGAUAspGGUGlyGUCGCCGACGGCGUAGCAGAAGluGGAGUGGCGGAGGGGUCACUUA

The central dogma of life includes the following processes: Replication: In this process, the DNA…

Answered: When researchers obtain genomic…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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he Sequence below comes from the alpha-2 globin of the human hemoglobin gene cluster found in chromosome 16. The globin region of the hemoglobin protein itself consists of 2 alpha chains and 2 beta chains. 1 actcttctgg tccccacaga ctcagagaga acccaccatg gtgctgtctc ctgccgacaa 61 gaccaacgtc aaggccgcct ggggtaaggt cggcgcgcac gctggcgagt atggtgcgga 121 ggccctggag aggatgttcc tgtccttccc caccaccaag acctacttcc cgcacttcga 181 cctgagccac ggctctgccc aggttaaggg ccacggcaag aaggtggccg acgcgctgac 241 caacgccgtg gcgcacgtgg acgacatgcc caacgcgctg tccgccctga gcgacctgca 301 cgcgcacaag cttcgggtgg acccggtcaa cttcaagctc ctaagccact gcctgctggt 361 gaccctggcc gcccacctcc ccgccgagtt cacccctgcg gtgcacgcct ccctggacaa 421 gttcctggct tctgtgagca ccgtgctgac ctccaaatac cgttaagctg gagcctcggt 481 agccgttcct cctgcccgct gggcctccca acgggccctc ctcccctcct tgcaccggcc 541 cttcctggtc…
When the cDNA was sequenced by the Sanger method utilizing ddCTP, the following products were obtained: Tetranucleotide Hexanucleotide Nonanucleotide Decanucleotide Dodenucleotide Octadecanucleotide Nonadecanucleotide 21-nucleotide 6c. What is the sequence of the bases in the mRNA coding for the peptide above? The
The following DNA strand is a template strand (also called non-coding strand) of an E. coli gene. Arrow indicates the direction of transcription. The asterisk indicates t transcription initiation site GCAGTGACCGGATATAACGAAGAGGAATGCCGTACAAA 5' GCAGTGA 3' -10 Which of the following sequences accurately represents the RNA derived from this gene? C5' UCCUUAC 3' C3' UCCUUAC 5' C5' CGUCACU 3' +10 3' AGGAATG 5'
In a genome project, the following genomic DNA sequences were obtained. Assemble the sequences into a contig. Using the assembled sequence, perform a BLASTn search. Does the search produce sequences similar to your assembled sequence? 5’ TCGGGGTCCTGGGATCTCATCACTGCAGCGC 3’ 5’ACTGCAGCGCTTTCCCAGCGGGCGGTGGTAC 3’ 5’GGGCGGTGGTACTCGGGAAGTCAGGAGTGTT 3’ 5’AGGAGTGTTTAAAACCTGGGGACTGGTTTTG 3’ 5’TGGTTTTGGGGGCGCTGAAGGCAGCGCAGGA 3’
The template strand of a gene has the sequence 5'-ATGCCTAGCCTAGGACT-3'. What will the sequence of the transcript from this gene be? 5' AGUCCUAGGCUAGGCAU 3 5 ATGCCTAGCCTAGGACT 3' 5 AUGCCUAGCCUAGGACU 3' 5' MAUGCCUAGCCUAGGACUAAAAAAAAAA 3" 5' AGTCCTAGGCTAGGCAT 3" V.
For the anticodon sequences 5' IAA and 5' xm^3s^2UAA, considering the DNA sequences of the genes encoding the tRNAs(assuming both tRNAs exist even if that is not true), What is the sequence of the RNA-like strand of each tRNA gene that corresponds to the tRNA's anticodon? be sure to indicate polarities.
Briefly discuss (referring to the images provided) why mutant 2 fails to produce functional protein. Note that none of the mRNA transcribed from this gene is of the expected size; some of the mRNA molecules produced are 223 nucleotides shorter than expected, whilst others are 47 nucleotides longer than expected.
This is the pre-mRNA of a mammalian gene with the spice sites marked. 5’-AGCUUCGCGUAAAUCGUAG/GUAAGUUGUAAUAAAUAUAAGUGAGUAUGAUAG\GGCUUUGG ACCGAUAGAUGCGACCCUGGAG/GUAAGUAUAGAUAAUUAAGCACAG\GCAUGCAGGGAUAUCCU CCAAAUAG/GUAAGUAACCUUACGGUCAAUUAAUUAG\GCAGUAGAUGAAUAAACGAUAU CGAUCGGUUAG/GUAAGUCUGAU-3’ This is the spliced mRNA with the spliced sites marked with a vertical line, “|”. 5’-AGCUUCGCGUAAAUCGUAG|GGCUUUGGACCGAUAGAUGCGACCCUGGAG|GCAUGCAGGGAUAUCCU CCAAAUAG|GCAGUAGAUGAAUAAACGAUAUCGAUCGGUUAGGUAAGUCUGAU-3’ Translate this mammalian mRNA into the correct amino acid sequence. In your answer, use the three letter abbreviations for each amino acid.
A lilP mutant called lilPXS is isolated that produces a truncated polypeptide of only 6 AA in length. Describe a single basepair DNA change that would lead to this truncated version of the protein. Multiple options are possible（100 words maximum）
Consider the following sequence fragment of an mRNA. Which of the miRNAS below would be competent for gene silencing? 5'-AUGCAAGCAUUGGCCAAGCUU-3' 5'-AUGCAAGCAUUGGCCAAGCUU-3 5'-UACGUUCGUAACCGGUUCGAA-3' 5'-AAGCUUGGUUAAUGCUUGCAU-3' 5'-UUCGAACCAAUUACGAACGUA-3' 3'-UUCGAACCAAUUACGAACGUA-5 3'-AUGCAAGCAUUGGCCAAGCUU-5'
Indicate the amino acid sequence of the protein encoded by the following mRNA molecule. Use the genetic code table and assume that the very first “AUG” the ribosome encounters will serve as the start codon and specify methionine. 5’-AAUUCAUGCCCAAAUUUGGGGCACGAAGCUUCUUAGGCUAGUCCUAAAAAA-3’
Write out the sequences of the two conserved elements in the following bacterial promoter. The startpoint of transcription is shown in red. TGCTTGACTCTGTAGCGGGAAGGCGTATTATGCACACCGCG

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