What proportion (in %) of the CFTR gene/DNA sequence is represented in the CFTR mRNA? The mRNA contains a 5’ UTR, ORF, and 3’ UTR. What proportion (in %) of the mRNA is represented in the ORF? **Title: Understanding the Expression of CFTR: A Step-by-Step Guide**The figures below depict the steps in the expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). Using the information in the figure, explore the detailed process below.### 1. Gene Structure- **CFTR gene (~190,000 base pairs):** The gene consists of exons and introns, which are segments crucial for the coding and non-coding regions of the mRNA.### 2. Transcription- **CFTR mRNA (6,132 nucleotides):** Following transcription, mRNA is formed with specific regions: - **5' UTR (132 nucleotides):** Untranslated region at the start of the mRNA. - **Translated region – ORF (4,443 nucleotides):** The open reading frame (ORF) that is translated into protein. - **3' UTR (1,557 nucleotides):** Untranslated region at the end of the mRNA.### 3. Translation- **Primary structure of CFTR protein (1,480 amino acids):** The sequence showing the location of major domains including transmembrane domains (TMD1 and TMD2), nucleotide binding domains (NBD1 and NBD2), and regulatory (R) domain.### 4. Protein Folding and Membrane Integration- **Folding into tertiary structure and embedding in cell membrane:** - **TMD1 and TMD2:** - Transmembrane domains, each with segments (1–6 and 7–12 respectively) forming part of the CFTR channel. - **NBD1 and NBD2:** - Nucleotide binding domains on the cytoplasmic side of the membrane. - ATP hydrolysis occurs here, crucial for changing the conformation of CFTR to function as an ion channel. - **ICLs and ECLs:** - Intracellular loops (ICLs) and extracellular loops (ECLs) connect transmembrane segments and aid in CFTR function. - **R domain:** - Regulatory domain where phosphorylation is necessary for CFTR to function effectively as an ion channel.This detailed pathway of CFTR expression explains both the genetic and molecular basis of its function, with implications for understanding conditions such as cystic fibrosis.

Name: What proportion (in %) of the CFTR gene/DNA sequence is represented in
Uploaded: 2024-03-20T06:42:41.000Z
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Description: What proportion (in %) of the CFTR gene/DNA sequence is represented in the CFTR mRNA? The mRNA contains a 5’ UTR, ORF, and 3’ UTR. What proportion (in %) of the mRNA is represented in the ORF? **Title: Understanding the Expression of CFTR: A Step-by-

The cystic fibrosis transmembrane conductance regulator (CFTR ) gene displays a tightly regulated tissue-specific and temporal expression. Mutations in this gene cause cystic fibrosis (CF), a common recessive lethal disorder. During transcription, the CFTR gene serves as a template for complementary base-pairing, in order to form a pre-mRNA molecule which contains both introns and exons. Pre-mRNA undergoes splicing of introns and is processed to form the mature mRNA. The total number of basepairs in CFTR gene is 190,000. The number of nucleotides in mRNA is 6132. Therefore, the proportion (in %) of the CFTR gene represented in the CFTR mRNA is: (6132/190,000) x 100 = 3.23% The open reading frame (ORF) is the part of a reading frame that has the ability to be translated. It is a continuous stretch of codons that begins with a start codon and ends at a stop codon. The total number of nucleotides in CFTR mRNA is 6132. The number of nucleotides in ORF is 4443. Therefore, the proportion (in %) of the mRNA represented in the ORF is: (4443/6132) x 100 = 72.46% Hence, The proportion (in %) of the CFTR gene represented in the CFTR mRNA is 3.23% The proportion (in %) of the mRNA represented in the ORF is 72.46%.