What is the purpose of adding table salt (NaCl) to the DNA extraction buffer? 1.Table salt dissociates into Na+ and Cl- in water The Na+ binds to negatively charged DNA and proteins, while Cl- binds to positively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer. 2.Table salt (NaCl) dissociates into Na+ and Cl- in alcohol. The Na+ binds to positively charged DNA and proteins, while Cl- binds to negatively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer. 3.Table salt (NaCl) dissociates into Na+ and Cl- in water. The Na+ binds to negatively charged DNA and proteins, while Cl- binds to positively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the alcohol layer. 4.Table salt dissociates into Na+ and Cl- in water. The Na+ binds to positively charged DNA and proteins, while Cl- binds to negatively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer.
What is the purpose of adding table salt (NaCl) to the DNA extraction buffer?
1.Table salt dissociates into Na+ and Cl- in water The Na+ binds to negatively charged DNA and proteins, while Cl- binds to positively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer.
2.Table salt (NaCl) dissociates into Na+ and Cl- in alcohol. The Na+ binds to positively charged DNA and proteins, while Cl- binds to negatively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer.
3.Table salt (NaCl) dissociates into Na+ and Cl- in water. The Na+ binds to negatively charged DNA and proteins, while Cl- binds to positively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the alcohol layer.
4.Table salt dissociates into Na+ and Cl- in water. The Na+ binds to positively charged DNA and proteins, while Cl- binds to negatively charged proteins, including histones, thus facilitating the separation of DNA from associated proteins, and also keeping proteins in the aqueous layer.
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