B. In the Meselson and Stahl experiment, both strands of the DNA double helix were labeled with a heavy isotope of Nitrogen (1®N) at the beginning (round 0). In subsequent rounds of replication, all new DNA is made with a light isotope of Nitrogen (“N). Isotopes have the same number of protons (the same chemical reactivity) but different numbers of neutrons and thus different atomic masses. Fill in the table on the next page with your predictions of the percentage of the double stranded DNA that would contain exclusively 1ºN, exclusively "N, or a mix of “N and tN after each round of replication. rounds of replication % DNA containing predictions for 3 proposed mechanisms of replication semi- conservative 0% 0% 100% conservative dispersive only 14N a mix of 14N and 15N only 1ºN only 14N a mix of 14N and 15N 1 |only 1ºN only 1“N a mix of 14N and 15N only 15N Concept check: What percentage of the DNA molecules have the heavy "N isotope at the beginning of the experiment (cycle 0)? Which models CANNOT be distinguished after just 1 round of replication? Where is this Nitrogen found?.
B. In the Meselson and Stahl experiment, both strands of the DNA double helix were labeled with a heavy isotope of Nitrogen (1®N) at the beginning (round 0). In subsequent rounds of replication, all new DNA is made with a light isotope of Nitrogen (“N). Isotopes have the same number of protons (the same chemical reactivity) but different numbers of neutrons and thus different atomic masses. Fill in the table on the next page with your predictions of the percentage of the double stranded DNA that would contain exclusively 1ºN, exclusively "N, or a mix of “N and tN after each round of replication. rounds of replication % DNA containing predictions for 3 proposed mechanisms of replication semi- conservative 0% 0% 100% conservative dispersive only 14N a mix of 14N and 15N only 1ºN only 14N a mix of 14N and 15N 1 |only 1ºN only 1“N a mix of 14N and 15N only 15N Concept check: What percentage of the DNA molecules have the heavy "N isotope at the beginning of the experiment (cycle 0)? Which models CANNOT be distinguished after just 1 round of replication? Where is this Nitrogen found?.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:B. In the Meselson and Stahl experiment, both strands of the DNA double helix were labeled with a heavy
isotope of Nitrogen (1®N) at the beginning (round 0). In subsequent rounds of replication, all new DNA is made
with a light isotope of Nitrogen (“N). Isotopes have the same number of protons (the same chemical
reactivity) but different numbers of neutrons and thus different atomic masses.
Fill in the table on the next page with your predictions of the percentage of the double stranded DNA that would
contain exclusively 1ºN, exclusively "N, or a mix of “N and tN after each round of replication.
rounds of
replication % DNA containing
predictions for 3 proposed mechanisms of replication
semi-
conservative
0%
0%
100%
conservative
dispersive
only 14N
a mix of 14N and 15N
only 1ºN
only 14N
a mix of 14N and 15N
1
|only 1ºN
only 1“N
a mix of 14N and 15N
only 15N
Concept check: What percentage of the DNA molecules have the heavy "N isotope at the beginning of the
experiment (cycle 0)?
Which models CANNOT be distinguished after just 1 round of replication?
Where is this Nitrogen found?.
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