What is the meaning of proofreading activity ?O A. The polymerase checks for the correct incorporation of nucleotides at the 5'end of the chainB. The polymerase checks for the correct incorporation of nucleotides at the 3'end of the growing chainO C. The polymerase does not attach to an unspecific primer binding to the templateO D. The polymerase is highly resistant to high temperatures, showing a prolonged half lifeO E. The Taq polymerase does not binds to the primer dimers

DNA means deoxyribo nucleic acids. DNA act as genetic material in most organisms present on earth.…

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Primer designing: A single-stranded DNA sequence (963 nucleotides) that codes for a hypothetical protein are shown below (lower case shaded blue). 1. Design a pair of forward and reverse primers (~18 nucleotides long each) with EcoRI and BamHI added at 5' and 3' ends, respectively, for the amplification and cloning of this a plasmid with the same restriction sites. gene into GTATCGATAAGCTTGATATCGAATTCatggctaaaggcggagct cccgggttca aagtcgcaat acttggcgct gccggtggcattggccagccccttgcgatgttgatgaagatgaatcctctggtttctgttctacatctatatgatgtagtcaatgcccctggtgtcaccgctgatatta gccacatggacacgggtgctgtggtgcgtggattcttggggcagcagcagctggaggctgcgcttactggcatggatcttattatagtccctgcaggtgttcctcg aaaaccaggaatgacgagggatgatctgttcaaaataaacgcaggaattgtcaagactctgtgtgaagggattgcaaagtgttgtccaagagccattgtcaacctg atcagtaatcctgtgaactccaccgtgcccatcgcagctgaagttttcaagaaggctggaacttatgatccaaagcgacttctgggagttacaatgctcgacgtagt cagagccaatacctttgtggcagaagtattgggtcttgatcctcgggatgttgatgttccagttgttggcggtcatgetggtgtaaccatttgccccttctatctcagg…
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An optimum ligation reaction should contain approximately 50 ng of vector. Given yourconcentrations recorded below, what is the volume of vector that should be added to eachligation reaction to have this mass of DNA in the reaction? Size of asPink-promoterless in bp: 702 (vector) Size of pCusC in bp: 157 The concentration of digested & purified PCR insert: 13.849 The concentration of digested and purified plasmid: 6.887 Any help with this is appreciated. I'm a bit confused thx
How does that length compare to that of restriction enzymes? Implications? 2. can you conceive of any potential applications of CRISPR technology?
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Please answer both parts, a. The enzyme that catalyzes the joining of fragments to form recombinant DNA is: Question 21 options: DNA polymerase DNA ligase helicase restriction endonuclease b. In the above diagram, ‘1’ represents the Question 3 options: sticky ends restriction sites primer restriction fragments
Replicating dna polymerases have high fidelity A why is high fidelity more importsnt for dna polymerase than rna polymerase b list 2 mechanisms dna polymerase 3 used to reduce errors c despite high fidelity dna polymerase makes errors which type of sequence leads to strands slippage what what types of mutations occur ( transversion, insertion, deletion, transition ) when slippage occurs
Give typed full explanation
5
Preparing plasmid DNA (double stranded, circular) for Sanger sequencing involves annealing a complementary, single-stranded oligonucleotide DNA primer to one strand of the plasmid template. This is routinely accomplished by heating the plasmid DNA and primer to 90°C and then slowly bringing the temperature down to 25°C. Why does this protocol work? What enzyme is used and what other components are required in the sequencing reaction? How does the Sanger method determine the sequence?
Suppose that you want to assay reverse transcriptase activity. If polyriboadenylate is the template in the assay, what should you use as the primer? Which radioactive nucleotide should you use to follow chain elongation?
DNA polymerase error rates can be 10-8 to 10° per nucleotide, and mutations in the repair functions of DNA polymerases that increase error rates can cause reduced survival phenotypes. Yet RNA polymerase error rates can be as high as 3 x105. Why is it so much more important for DNA polymerase to be accurate than RNA polymerase? Be brief.

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