A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction. This technique detects the fluorescence produced by reporter molecules like SYBR® Green dye or TaqMan® probe during the DNA amplification reaction. Compare and contrast between SYBR® Green and TaqMan® probe with illustrations.
Q: You cannot get a cavity, tetnus, or sinus infection from another person. This means that all of…
A: Tetanus is a disease of nervous system which is caused by a toxin producing bacteria. This disease…
Q: What do the terms “chains, clusters, pairs, and tetrads” refer to in the arrangement of bacteria?
A: Bacteria generally multiply via binary fission and results in two identical daughter cells. These…
Q: Draw a bracketed phylogenetic tree of the following groups on the next blank page: Peat Moss…
A: Phylogenetic tree with related traits
Q: Determine if the cells in prophase of mitosis are haploid or diploid.
A: Mitosis is divided into four stages: prophase, metaphase, anaphase, and telophase. The longest phase…
Q: Why is it essential to balance your 400 mL of cell culture before centrifuging?
A: Ans: A centrifuge is a machine which use centrifugal force to separate the components of a mixture.…
Q: What is being separated during anaphase I of meiosis? What happens to the sister chromatids during…
A: Please follow step 2 for detailed explanation.
Q: A) What module can we use to run BLAST over the internet in Biopython: Bio.Blast.NCBIWWW…
A: Biopython is a popular application programming interface (API) containing free tools, and are…
Q: Make Abstract about parasitic crusteceans of farmed fish
A: Parasitic crustaceans are those which infect fishes and shell fishes. It belong to the group of…
Q: What is color opponency and how is it coded in the retina?
A: The human visual system decodes colour information by processing photoreceptor cell impulses in an…
Q: Macmillan Learning Hominin is a group of species that includes modern humans and extinct human-like…
A: INTRODUCTION Primates : primates are mammal group including monkeys, apes and human.
Q: of three cases of hemolytic uremic syndrome (HUS) among children caused by Escherichia coli O157:H7…
A: Hemolytic uremic syndrome is life threatening sometimes. The symptoms are diarrhoea (bloody), fever,…
Q: List other diseases that are associated with increased levels of plasma lipids
A: Fatty, waxy, or oily compounds are referred to as lipids. They are soluble in organic solvents but…
Q: 6. A man with type B blood marries a woman with type A blood. They have the first child with blood…
A: Blood group A and B are dominant over blood group O. Blood grouping show codominance.
Q: What does your map of cutaneous sensations tell you about the distribution of sensory receptors in…
A: Introduction - Skin - heaviest organ in the body- Protects the organism by keeping damaging agents…
Q: Fish from Group Number 1 2 3 4 5 6 7 8 9 10 Average: Std Isotocin Injected Control # of acts per 10…
A: Due to little progress was being made in understanding aggression and violence, particularly among…
Q: An anticodon strand reads 5'-IAG-3'. Fill in the missing base sequences for the possible codons…
A: The anicodon is 5' IAG 3' so the codon for this anticodon would be 5' TGC 3' if the I were to be…
Q: Baltimore Oriole Black-backed Oriole Female Male Kondo, Baker, & Omland 2004 2B. Based on the…
A: The Baltimore oriole is a small icterid blackbird that is common in eastern North America as a…
Q: To maintain muscle mass, in-season training should rotate between maximal strength and power…
A: Simply said, muscle mass refers to the total quantity of skeletal, smooth, and cardiac muscles in…
Q: Which amino acid has a sulfur atom in its side chain and can form a disulfide bridge?
A: Introduction Amino acids are essential elements of the peptide sequences. Longer peptide sequences…
Q: missing a name or two from the list (Enterobacter aerogenes), and (Serratia rubidaea)- should be 20…
A: Biochemical testing and staining procedures are used to identify bacteria. For example, in substrate…
Q: Which of the following individuals would you expect to share 25% of their DNA with you? Select all…
A: Answer :- Your aunt or uncle Your grandparents Your Siblings These are the one which are the…
Q: What are the THREE (3) important regions in all plasmids? Explain their functions.
A: Introduction :- A plasmid is a discrete piece of extrachromosomal DNA that is physically distinct…
Q: Determine if prophase II of meiosis contains 2 chromatids or only one chromatid.
A: A single cell divides twice during the meiotic process to produce four haploid daughter cells. The…
Q: Andi worked on a construction site for part of the time that she was pregnant with her daughter,…
A: Que 1- Is Abbi's brain affected by the lead because lead is harmful for baby's brain development…
Q: What variables can affect the transformation efficiency?
A: Introduction The ability of cells to absorb extracellular DNA and express the genes that are…
Q: Considering the anatomy of reptiles and amphibians explain the difference between these two 2…
A: Reptiles and amphibians are the important organisms under the animal kingdom. They are tetrapods…
Q: the TPOX allele with the structure [TAGA]10 will be ______ nucleotides longer than the allele with…
A: [TAGA]10 will have four nucleotides longer than the allele with the structure [TAGA]5
Q: The increase in fast food establishments on Kirkwood Avenue is taking a deadly toll on the squirrels…
A: Cholesterol A waxy, fat-like substance that the body requires in small amounts for good health.…
Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…
A: Removing RNA is one of the crucial processes in plasmid purification, especially after the manual…
Q: Genetic drift: is variation in the relative frequency of different genotypes in a small population,…
A: Genetic drift It is mechanism of evolution, responsible for random changes in the gene pool. It…
Q: Which of the following gene regulatory molecules act in trans. Check all that apply. The lac…
A: Introduction:- The gene regulatory molecules helps in regulating the expression of different genes,…
Q: METAPHASE CELLS: Find and observe a metaphase cell. Sketch or paste it on the right ANAPHASE: Find…
A: Mitosis and meiosis are the two distinct processes of cell division. When we talk about "cell…
Q: Where does hematopoiesis take place? spongy bone compact bone osteons outer…
A: Hematopoiesis can be defined as a process through which formation of all types of blood cells takes…
Q: JE V plasma membrane capsule nucleoid fimbriae ribosome 0 G 0
A: Prokaryote word is derived from "pro" means primitive, and "karyon" means nuclear membrane.…
Q: Among the following statements regarding the use of viral vectors, which is (are) true: (can be more…
A: Transfer of foreign nucleic acid insert into the eukaryotic cell is transfection. For many…
Q: Human cells have a diploid number of 46. How many chromosomes does a human cell have during anaphase…
A: Cell division is a phenomenon during which splitting of parent cell results in formation of new…
Q: The modern human skull is: Select an answer and submit. For keyboard navigation, use th a The one…
A:
Q: Recommend an international strategy for Drip Footwear to consider
A: Introduction: Drip Footwear is not merely a brand of sneakers. It is a company whose mission is to…
Q: Bone tissue can be described as a. dead calcified tissue b. cartilage c. the skeletal system d.…
A:
Q: Vitamin list: Vitamins may be used more than once Thiamin Folate Riboflavin Vitamin B12 Niacin…
A:
Q: What increases/decreases intercellular co2 (Ci) and how does it correlate with assimilation rates?
A: Photosynthesis is the process by which green plants take up light and transform it into the chemical…
Q: Which of the following specifically explains why glucose uptake into intestinal cells happens in…
A: Absorption is the process by which the end products of digestion pass through the intestinal…
Q: Construct a cladogram for: Raccoon, Arctic Fox, Armadillo, Sheep, Snow Leopard, Hippopotamus,…
A: Cladogram A diagram that depicts species relationships. These connections are founded on observable…
Q: 43. Which of the following produced a nonvolatile acid? a. high nitric oxide levels b. emphysema c.…
A: Nonvolatile acid is metabolic acid produced by the metabolism of fat, carbohydrates, or proteins.…
Q: WHO is Henry Lowe ? what what did he created?
A: Dr Henry Lowe was born on April 9, 1939. He is a scientist and businessman in the health industry.…
Q: how may high blood levels of cholesterol be reduced
A: Cholesterol is a type of sterol, which is a type of lipid. Lipids are oily compounds which are…
Q: I. Growth of mold colony. Fill the table with the desired information Colony Characteristics Color…
A: Moulds include all species of the microscopic fungi that grow in the form of multicellular…
Q: A B cat frame-shift no cat no cat cat nonsense cat C % survival 120 100 80 60 40 20 0 42 cat cats…
A: Fig 4: Panel A- Technique: Fluorescence microscopy has been used. All the 4 strains are grown on in…
Q: There are several lines of evidence that suggest that chloroplasts and mitochondria were once…
A: For performing independent life, energy production is very much important. Some organisms depend on…
Q: 22. VIRAMUNE Oral Suspension contains 1% w/v of nevirapine. Calculate the milli- grams of nevirapine…
A: VIRAMUNE: Nevirapine (NVP), marketed among other names as Viramune, is a drug intended to treat and…
Step by step
Solved in 2 steps with 2 images
- Examine the DNA fragment sequence below. Your job is to design primers for PCR that would be able to amplify this DNA fragment. Design the primers so that they are 7 bases in length. Don’t forget to indicate direction (polarity) of the primers. Also describe where the primer would bind (i.e. top or bottom strand, left or right side of the DNA strand). Please organize your response so that each primer, and associated information, is separated by at least one blank line 5’ - TCCACTTGCTGTGTAGCTAAATCATATAACAG3’ - AGGTGAACGACACATCGATTTAGTATATTGACWhy is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…Explain why DNA ladders are usually included during gel electrophoresis. One aspect of PCR that can be modified is the annealing temperature. In general, higher annealing temperatures show more specificity towards a single template, whereas lower annealing temperatures show less specificity and may bind to multiple regions throughout the genome. Discuss how using an annealing temperature that is too high or too low might influence the results of a PCR assay (and gel electrophoresis results) such as the one used in this study.
- Labelled primers or dNTPs are useful in some recombinant DNA techniques. What are DNA primers? What labelled primer can be incorporated into DNA primers. Describe the recombinant DNA techniques where these labelled primers or dNTPs are used.During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityList the steps in the polymerase chain reaction; discuss one disadvantage to this technique
- Discuss the following statement: “primase is a sloppy enzyme that makes many mistakes. eventually, the rna primers it makes are disposed of and replaced with dna synthesized by a polymerase with higher fidelity. this is wasteful. it would be more energy-efficient if a dna polymerase made an accurate copy in the first place.”An optimum ligation reaction should contain approximately 50 ng of vector. Given yourconcentrations recorded below, what is the volume of vector that should be added to eachligation reaction to have this mass of DNA in the reaction? Size of asPink-promoterless in bp: 702 (vector) Size of pCusC in bp: 157 The concentration of digested & purified PCR insert: 13.849 The concentration of digested and purified plasmid: 6.887 Any help with this is appreciated. I'm a bit confused thxK What is an example of positive feedback? sweating when you are hot increased respiration during exercise decreased blood glucose levels when insulin is released maintenance of blood pH levels labour contractions during birth
- For what purposes is DNA extraction done? (give at least 3 purposes for which you may need to extract DNA)The exponential nature of PCR allows spectacular increases in the abundance of a DNA sequence being amplified. Consider a 10-kbp DNA sequence in a genome of 1010 base pairs. What fraction of the genome is represented by this sequence; i.e., what is the fractional abundance of this sequence in this genome? Calculate the fractional abundance of this target sequence after 10, 15, and 20 cycles of PCR, starting with DNA representing the whole genome and assuming that no other sequences in the genome undergo amplification in the process.In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is the purpose of this step? In DNA isolation from blood samples, why does the vial for blood storage contain EDTA? In the preparation for DNA isolation in plants, the plant source is refrigerated and ground prior to extraction. Why is this so? Why are DNA pellets air-dried before resuspension in buffer?