A real-time polymerase chain reaction is a laboratory technique of molecular biology basedon the polymerase chain reaction. This technique detects the fluorescence produced byreporter molecules like SYBR® Green dye or TaqMan® probe during the DNA amplificationreaction. Compare and contrast between SYBR® Green and TaqMan® probe withillustrations.

SYBR TAQMAN Not so costly Expensive Easy to use Difficult to use Binding the fluorescent dye…

Answered: A real-time polymerase chain reaction…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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A real-time polymerase chain reaction is a laboratory technique of molecular biology based
on the polymerase chain reaction. This technique detects the fluorescence produced by
reporter molecules like SYBR® Green dye or TaqMan® probe during the DNA amplification
reaction. Compare and contrast between SYBR® Green and TaqMan® probe with
illustrations.

Expert Solution

Step 1

SYBR	TAQMAN
Not so costly	Expensive
Easy to use	Difficult to use
Binding the fluorescent dye to double-stranded deoxyribonucleic acid (dsDNA)	Dual labeled oligonucleotide and exonuclease activity of Taq polymerase enzyme.
Low Specific	Highly specific
Optimization needed for real -time PCR	Does not need to optimize
Varibale senitivity	sensitivity : upto 1-10 copies

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