Answered: what experiment can be used to confirm…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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what experiment can be used to confirm that agglutination was due to the binding of the lectins to sialic acid.

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Introduction

Lectin is the collective name for all sugar-binding proteins. Lectins have one or more than one binding site for glycans.

Sialic acid is a 9-carbon alpha-keto acid often found in the terminal position in the glycan moiety of glycoproteins. Sialic acid is linked to the hydroxyl group on carbon 3 or carbon 6 of galactose or to the carbon 6 hydroxyl group of N-acetylglucosamine or N-acetylgalactosamine residues. These residues are found on cell surfaces as either glycoproteins or glycolipids where they play an important role in cell recognition.

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