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Which of the following best describes the production of insulin in by bacterial cells The hinsynt

Which of the following best describes the production of insulin in by bacterial cells The hinsynt

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Which of the following best describes the production of insulin in by bacterial cells. The biosynthetic pathway that produces human insulin is introduced into bacterial cells on a plasmid. Insulin is purified from bacterial lysates by electrophoresis. Human insulin in a peptide hormone comprised of two chains A and B. Only B chain is required, It is produced by injecting bacteria with MRNA encoding B chain peptide, and purifying the peptides with an antibody. Fuman insulin in a peptide hormone comprised of two chains A and B. It is produced by injecting bacteria with transgenic plasmids containing MRNA encoding either the A and B chain peptides, and purifying the peptides with a mix of antibodies against tne A or B chain. aman nsulina peptide hormone comprised of two chains A and B. They are produced separately as fusion proteins with beta-galactosidase when bacterial cells are transformed with plasmids containing either A or B chain with the lacZ sequence. Once purified they are cleaved from beta-galactoside and mixed together to produce a functional hormone. Question 29 of 33>> A Moving to another question will save this response.
Transcribed Image Text:Which of the following best describes the production of insulin in by bacterial cells. The biosynthetic pathway that produces human insulin is introduced into bacterial cells on a plasmid. Insulin is purified from bacterial lysates by electrophoresis. Human insulin in a peptide hormone comprised of two chains A and B. Only B chain is required, It is produced by injecting bacteria with MRNA encoding B chain peptide, and purifying the peptides with an antibody. Fuman insulin in a peptide hormone comprised of two chains A and B. It is produced by injecting bacteria with transgenic plasmids containing MRNA encoding either the A and B chain peptides, and purifying the peptides with a mix of antibodies against tne A or B chain. aman nsulina peptide hormone comprised of two chains A and B. They are produced separately as fusion proteins with beta-galactosidase when bacterial cells are transformed with plasmids containing either A or B chain with the lacZ sequence. Once purified they are cleaved from beta-galactoside and mixed together to produce a functional hormone. Question 29 of 33>> A Moving to another question will save this response.
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