The section shown above is from mouse pancreas, which has been stained using an immunocytochemistry protocol against glucagon. Which of the following are the cells stained red?
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![The section shown above is from mouse pancreas, which has been stained using an immunocytochemistry
protocol against glucagon. Which of the following are the cells stained red?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa88e1f72-0527-4c98-9df1-6ef754818e35%2Fd52abf0c-54e0-4bac-9246-9a0e1e869471%2Fszn9fnn_processed.png&w=3840&q=75)
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- Identify three other methods commonly used to visualize lipids on a TLC plate. Specify the type or class of lipids that are detected using these methods.Name the cells in the islets of langerhans which make glucagon and summarize the principles and procedures specific to how these cells were identified in immunostaining. word limit 250Why is stimulating cells with LPS done for Splenocyte Preparation?
- An injection contains 5 million international units (MIU) of interferon alpha-n3 (ALFERON N) proteins per milliliter. The recommended dose is 0.05 mL. The literature states that the activity of interferon alpha-n3 is approximately equal to 2.6 × 108international units/mg of protein. Calculate (a) the number of international units and (b) the micrograms of interferon alfa-n3 proteins administered per dose.Can a mouse infected with Bacillus anthracis generate antibodies against the S-layer? How do you know? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/You are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodamine
- Several classes of LDLreceptor mutations have been identified as causes of this disease. Suppose that you have been given cells from patients with different mutations, an antibody specific for the LDL receptor that can be seen with an electron microscope, and access to an electron microscope. What differences in antibody distribution might you expect to find in the cells from different patients?Mannose binding lectins (MBL) and ficolins are the two classes of proteins that can initiate the lectin pathway of complement activation. These proteins are selective for activating complement on the surfaces of microbial pathogens rather than host cells because: Their higher-order oligomeric structure can be assembled only after the monomers first bind to pathogen membranes. They only recruit MASP (MBL-associated serine proteases) proteins when bound to pathogen surfaces and not when bound to host cells. They only undergo the conformational change needed to activate MASP proteins when bound to a pathogen and not when bound to a host cell. They only bind to carbohydrate side chains and oligosaccharide modifications found on pathogen surfaces but not on host cell membranes. The activated MASP proteins are rapidly inactivated by hydrolysis when present on the surface of a host cell.Is this statement is correct or incorrect. Explain with reason and related physiological concepts. Pattern recognition receptors (PRRs) can be found on the plasma membrane of macrophages.
- True/False: Neutrophils regulate the production of active cathelicidins (a class of antimicrobial peptides) by segregating the inactive propeptide from the processing enzyme that cleaves and activates it in two different types of cytoplasmic granules. These two types of granules are induced to fuse with phagosomes after ingestion of microbes, bringing the processing enzyme and the propeptide together.Complement activation is a cascade reaction, with each component sequentially acting onothers, in a similar way to the blood-clotting system. Discuss the effects that occur whenpeptides are generated from the activation by either the classical or the alternative pathwayNeutralizing antibodies are effective at preventing infection or toxicity mediated by pathogens or their toxic products. In fact, nearly all vaccines currently in use function by eliciting neutralizing antibodies. One example is the tetanus vaccine, in which neutralizing antibodies are generated against an inactivated form of the tetanus toxin (the tetanus toxoid). The most important feature of a neutralizing antibody is having high affinity for the antigen. being efficient at activating the complement cascade. having a high degree of multivalency, such as being a pentamer or hexamer of immunoglobulin monomers. being present at a high concentration in the circulation. 0 0 0 0