Labs that do a lot of Western blots often have hundreds of primary bodies but only two or three secondary antibodies. Why? I understand that secondary antibody aids in the detection, sorting or purification of target antigens by binding to the primary antibody which directly binds to the target antigen. However, I don't undrstand why there are only two or three secondary antibodies available in the laboratory. Glad if the expert would advise.

Q: What will you find at the center of a clear plaque?

A: Plaques is opaque field of bacterial cultures which are infected by a virus or bacteriophage.

Q: Which of the following antigen-antibody reaction is used in blood ?grouping Precipitation O…

A: Blood is thick fluid that circulates through out the body in order to supply oxygen and nutrients to…

Q: To determine whether an enzyme-linked antibody is bound to an antigen, __________ must be added,…

A: ELISA is an enzyme linked immunosorbent Assay which is a biochemical test used to detect the…

Q: In immunoassays such as western blotting and ELISA the secone antibody binds to what part of the…

A: Antibodies are proteins that are important for our body to destroy pathogens like viruses, bacteria,…

Q: True or false:   RNA is conserved mechanism in eukaryotes. SDS is an uncharged detergent…

A: RNA or ribonucleic acid is the genetic material found in some living organisms, including some…

Q: Identify the highlighted part of the antibody Antigen Binding Site Constant region Constant region…

A: Antibodies are also refers to as Immunoglobulin ( Ig ) and are basically proteinaceous substances…

Q: direct fluorescent antibody, ouchterlony diffusion, ELISA) which do you think would work best for…

A: Blood grouping Blood grouping is the classification of the blood based on the type of antigen/s that…

Q: Antigen Antibody Enzyme-labelled antibody Substrate This image represents a/an. direc ELISA sandwich…

A: Enzyme-linked immunosorbent assay (ELISA) is an immunological assay that is used in laboratories to…

Q: antibodies in biotechnology?

A: Biotechnology is a wide range of studies where it uses its techniques with biological systems to…

Q: What do you mean by immunofluorescence

A: Antibodies are immunoglobulins produced in response to antigenic stimulation. They may either bound…

Q: How we can Treatment  Severe Combined Immunodeficiency (SCID) by using gene therapy? Please answer…

A: SEVERE COMBINED IMMUNODEFICIENCY:- SCID stands for severe combined immunodeficiency, a group of…

Q: Please help me illustrate the structure of ABH antigen substances

A: The ABO blood grouping system was discovered by Karl Landsteiner. A, B, AB, and O are the main blood…

Q: Which of the following is the correct pairing of an immunologic test and the subject to be…

A: Several immunological techniques help to diagnose the various infections to human body, major…

Q: Immunocompromised patients are at a higher risk of cancer. Discuss how the immune system plays a…

A: The immune system which recognises foreign microorganisms as nonself and mounts of response to…

Q: Explain how the Western blot and complement fi xation tests are performed.

A: Proteins are the most abundant organic molecule in living systems. It plays a role in structural,…

Q: True or false 1. Heterogenous antibodies used for structural studies can be obtained by monoclonal…

A: Heterogenous antibodies - Heterogenous terms indicates that the molecule is a hybrid. i.e. a single…

Q: Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus…

A: Answer.) :- To calculate the required cell density for the new culture, you can use the…

Q: Which statement is right the polyclonal antibody is expensive to produce the monoclonal antibody…

A: Antibodies are giant Y-shaped proteins known as immunoglobulins that are created by B cells as a…

Q: 60) An investigator is studying the immune response to an encapsulated strain of staphyloccoccus…

A: Introduction  The immune response is the body's way of defending itself against harmful substances,…

Q: Predict the outcome of an error in ELISA protocol consisting of adding the antibodies in the wrong…

A: ELISA stands for enzyme linked immunosorbant assay.This techniques is basically used for the…

Q: To detect a toxin in food, the contaminated food is normally injected into at least two guinea pigs.…

A: The Humoral immune response in the body is regulated by the B cells which produce antibodies against…

Q: Why we choose antibodies as a topic in my biotechnology presentation?  Please shortly answer at your…

A: The present world is dynamic, ever-changing. New technologies emerging every day and are presenting…

Q: HIV testing uses a(n) ________________ELISA. direct indirect

A: Antibody tests can take 23 to 90 days to identify HIV disease after exposure. Most quick tests and…

Q: A blood bank technologist working in an army medical center discovers that a colleague from boot…

A: The scenario describes a situation where a blood bank technologist discloses confidential medical…

Q: An ELISA test is useful for detecting the presence of antigens but not antibodies in a sample.…

A: The enzyme-linked immunosorbent assay (ELISA) test is used to diagnose HIV which causes AIDS. It is…

Q: In the description of the preparation of an OR for surgery, bleach rather that alcohol was used to…

A: The disinfectants are the products that are used to kill or eliminate or inactivate microorganisms…

Q: Difference between Antibody ELISA and Antigen ELISA

A: Introduction Enzyme-linked immunoassay (ELISA) is an acronym for enzyme-linked immunoassay.…

Q: MBS crosslinker contains aromatic ring in the spacer. discuss if this ring structure helps stabilize…

A: The presence of an aromatic ring in the spacer of a maleimide-based crosslinker can indeed…

Q: be 96% sensitive. What does that mean? A western blot was used on the same sample to confirm, and…

A: Enzyme linked immunosorbent assay is a very sensitive test used to detect and also quantify…

Q: True or false?  SDS PAGE gels can be probed with antibidies to perform an immuboblot.  C.elegans…

A: Biological science is a diverse field of study. It comprises numerous branches, for example,…

Q: kDa 188- 62- 49- 38- -heavy chain 28- -light chain 18- 14- 6- 3- 1 2

A: While casting a gel, well are created on the gel to load the samples. When protein samples run in a…

Q: Antibody heavy and light chain polypeptides consist of repeated domains, each of which is ~110 amino…

A: The immune system of the human body consists of specialized cells, proteins, tissues, and organs to…

Q: Anti-A antibodies are added to a blood sample to detect Anti-B antibodies are added to a blood…

A: Introduction :  There are some proteins in the blood called antibodies and antigens which differ…

Q: Bacteria In the petri dish in Figure 1, the bacteria Esherichia coli (E. coli) was grown and treated…

A: Bacteria are unicellular prokaryotic microorganisms (nucleus is not enclosed in a nuclear membrane)…

Q: Which of the following rapid testing strategies is a molecular test that detects a pathogen-specific…

A: Answer: Polymerase chain reaction (PCR) test is a molecular test that detects a pathogen-specific…

Q: Which technique is best for determing the the intracellular localization of a protein? Question…

A: Intracellular localisation of protein is the determination of the cellular components where protein…

Q: You extract B cells and dilute the cells so there’s one B cell in each well of a 10-well plate. You…

A: The ability of the body to defend itself against invading foreign agents like bacteria, viruses or…

Q: serology (elisa) lab: Why are enzymes used in this immunoassay?

A: Enzymes are biological catalysts. They speed up the rate of reaction. They decrease the activation…

Q: What is the immunoassay analyzer Machine

A: Immunoassay is a test that is used to measure the concentration of a macromolecule or a small…

Q: To detect the protein in Western blotting,     antibody is used     antigen is used     DNA probe…

A: Blotting is a separation technique, in which the samples of DNA, RNA, and proteins are immobilized…

Q: If C3b was prevented to bind to C5 convertase, would the complement system still illicit an immune…

A: The immune system is responsible for the protection against the pathogens. The white blood cells or…

Q: A patient is O positive and his serum is agglutinating all screening and panel reagent cells at room…

A: Blood group of the given patient is O positive. His serum is agglutinating all screening and panel…

Q: immunoassays are able to detect infections disease because of a very specific match between

A: The correct answer is 1. Antigen , Antibody

Q: Many vaccines are heat sensitive due to their high concentration of proteins. Which of the following…

A: A heat sensitive vaccine is one which if exposed to temprature above certain specific values will be…

Q: 29. Correctly label each image below with the ELISA method it is describing (3pts) Ag Ampan…

A: Introduction:- ELISA is a plate based assay technique designed for detecting and quantifying soluble…

Immune Disorders

The collaboration between the cells and proteins that work together as a defense system against infection comprises the immune system.Each of them performs a variety of tasks to recognize and/or react against a foreign body.