1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false 3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false
1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false
2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge true/false
3) PBST is a buffer that is used for blocking the membrane during immunoblotting. true/false
4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false
7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false
10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme
A) Alkaline decarboxylase
B) GAPDH
C) Alkaline Phosphatase
D) Horseeradish peroxide
13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein
A) SDS
B) 2- mercaptoethanol
C) Tris-HCL pH6.8
D) Tris- HCL PH 8.8
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