Use the plasmid to answer the following questions a) Digest the plasmid with EcoRI only and then both EcoRI and BamHi together. Fill in the table to show the fragment sizes produced.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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2. Use the plasmid to answer the following questions : [6T]
a) Digest the plasmid with EcoRI only and then both
EcoRI and BamHi together. Fill in the table to show the
fragment sizes produced. [3]
EcoRI only
EcoRI and
BamHI together
Fragmen
t sizes
produced
Add text
BamHI
5050 bp
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BamHI
0/6400 bp
3100 bp
830 bp
EcoRI
Highlig
EcoRI](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa039888e-1ae1-439f-9003-82d25305c4a4%2Fe3252045-3967-4a27-8265-320df87bfd01%2Fsmddnle_processed.jpeg&w=3840&q=75)
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