Use the information provided in the experiment below to annotate/ label the figure. Background: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by using moleFour Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (TO-34) containing one copy of crylla detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of crylla antibody was found in the midgut of boll weevils fed on TO-34 buds in an immunodetection assay. The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to TI progeny. All plants showed phenotypically normal growth, with fertile flowers and abundant seeds. Results relating to figure: In feeding bioassays, fall armyworm larvae and boll weevil adults were fed on tissues of TO-34 plants over a period of 7 days in order to estimate the mortality rates due to crystal ingestion. The results obtained show the tangible possibility of con- trolling fall armyworm and boll weevil in a non-chemical way. The mortality rates observed in entomological assays for fall armyworm (88.1%) and boll weevil (83.7%) fed on cotton leaves over a period of 7 days were high, but the mortality rate was moderate (56.7%)for boll weevilfed on floral buds (Table 3). This value is justified, given that the promoter used in our gene construction, CaMV 35S, has been reported to have broad expression in various plant organs but limited expression in reproductive structures. Seven lines have been identified with mortality rates close to those presented in Table 3 (data not given)
Genetic Variation
Genetic variation refers to the variation in the genome sequences between individual organisms of a species. Individual differences or population differences can both be referred to as genetic variations. It is primarily caused by mutation, but other factors such as genetic drift and sexual reproduction also play a major role.
Quantitative Genetics
Quantitative genetics is the part of genetics that deals with the continuous trait, where the expression of various genes influences the phenotypes. Thus genes are expressed together to produce a trait with continuous variability. This is unlike the classical traits or qualitative traits, where each trait is controlled by the expression of a single or very few genes to produce a discontinuous variation.
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