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Tube # 2c Table 3-Determination of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 3c 4c Q12) After 10 min at experimental temperature was enzyme activity high or low at 75°C and 100°C? Support your answer by looking at the difference between 2nd and 1st absorbances. ( 5c Q13) Was the enzyme denatured at 75°C and/or 100°C% Support your answer by looking at what happens after returning enzyme to room temperature AFTER enzyme was subjected to experimental temperatures of 20 °C, 40 °C,60 °C,80 °C and 100°C./¹ Q14) What happens to the enzyme structure and function when it becomes denatured? ( Potato Expt. extract Temp. (mL) 2 2 2 2 room temp 20 °C 40 °C dH₂O Catechol (mL) (mL) Boiling 100 °C 0 0 75°C 0 0 13 13 13 13 0 min. at Expt. Temp. Start Time: 4:20 Absorbance: 0.072 Absorbance: 0.114 Start Time: 25 Absorbance: 0.128 Start Time: 4:25 Time for reading: 4:33 Absorbance: 0.132 Start Time: 4:27 2nd Absorbance after 10 min. in Expt Temp. Absorbance: 0.097 Time for reading: 4:30 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.023 0.061 0.193 Time for reading: 4:37 Absorbance: 0-126 0.029 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43 Absorbance: 0.119 Time for reading: 4:45 Absorbance: 0-209 Time for reading: 4:47 Absorbance: 0.115 Subtract 2nd from 3rd absorbance 0.031 -0.018 10.016 -0.011

Tube # 2c Table 3-Determination of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 3c 4c Q12) After 10 min at experimental temperature was enzyme activity high or low at 75°C and 100°C? Support your answer by looking at the difference between 2nd and 1st absorbances. ( 5c Q13) Was the enzyme denatured at 75°C and/or 100°C% Support your answer by looking at what happens after returning enzyme to room temperature AFTER enzyme was subjected to experimental temperatures of 20 °C, 40 °C,60 °C,80 °C and 100°C./¹ Q14) What happens to the enzyme structure and function when it becomes denatured? ( Potato Expt. extract Temp. (mL) 2 2 2 2 room temp 20 °C 40 °C dH₂O Catechol (mL) (mL) Boiling 100 °C 0 0 75°C 0 0 13 13 13 13 0 min. at Expt. Temp. Start Time: 4:20 Absorbance: 0.072 Absorbance: 0.114 Start Time: 25 Absorbance: 0.128 Start Time: 4:25 Time for reading: 4:33 Absorbance: 0.132 Start Time: 4:27 2nd Absorbance after 10 min. in Expt Temp. Absorbance: 0.097 Time for reading: 4:30 Absorbance: 0.036 0-137 Time for reading: 4:35 Absorbance: Subtract 1st from 2nd absorbance 0.023 0.061 0.193 Time for reading: 4:37 Absorbance: 0-126 0.029 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43 Absorbance: 0.119 Time for reading: 4:45 Absorbance: 0-209 Time for reading: 4:47 Absorbance: 0.115 Subtract 2nd from 3rd absorbance 0.031 -0.018 10.016 -0.011

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Tube # 2c Table 3-Determination of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. 3c 4c Q12) After 10 min at experimental temperature was enzyme activity high or low at 75°C and 100°C? Support your answer by looking at the difference between 2nd and 1st absorbances. ( 5c Q13) Was the enzyme denatured at 75°C and/or 100°C% Support your answer by looking at what happens after returning enzyme to room temperature AFTER enzyme was subjected to experimental temperatures of 20 °C,40 °C,60 °C,80 °C and 100°C. "¹ /1 Q14) What happens to the enzyme structure and function when it becomes denatured? ( Potato Expt. extract Temp. (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C Boiling 100 °C dH₂O Catechol (mL) (mL) 0 0 0 0 13 || 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: 4:25 Absorbance: 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: Subtract 1st from 2nd absorbance 0.036 0-137 Time for reading: 4:35 Absorbance: 0-193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43 Absorbance: 0.119 Time for reading: 4:45 Absorbance: 0-209 Time for reading: 4:47 Absorbance: 0.115 port Material Subtract 2nd from 3rd absorbance 0.031 -0.018 0.016 -0.011
Transcribed Image Text:Tube # 2c Table 3-Determination of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. 3c 4c Q12) After 10 min at experimental temperature was enzyme activity high or low at 75°C and 100°C? Support your answer by looking at the difference between 2nd and 1st absorbances. ( 5c Q13) Was the enzyme denatured at 75°C and/or 100°C% Support your answer by looking at what happens after returning enzyme to room temperature AFTER enzyme was subjected to experimental temperatures of 20 °C,40 °C,60 °C,80 °C and 100°C. "¹ /1 Q14) What happens to the enzyme structure and function when it becomes denatured? ( Potato Expt. extract Temp. (mL) 2 2 2 2 room temp 20 °C 40 °C 75 °C Boiling 100 °C dH₂O Catechol (mL) (mL) 0 0 0 0 13 || 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: 4:25 Absorbance: 0.132 Start Time: 4:27 Absorbance: 0.097 2nd Absorbance after 10 min. in Expt Temp. Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: Subtract 1st from 2nd absorbance 0.036 0-137 Time for reading: 4:35 Absorbance: 0-193 Time for reading: 4:37 Absorbance: 0-126 0.023 0.061 0.029 Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: 0.159 Time for reading: 4:43 Absorbance: 0.119 Time for reading: 4:45 Absorbance: 0-209 Time for reading: 4:47 Absorbance: 0.115 port Material Subtract 2nd from 3rd absorbance 0.031 -0.018 0.016 -0.011
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