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What would be the controlled variables and the controls in this experiment if any?

What would be the controlled variables and the controls in this experiment if any?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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What would be the controlled variables and the controls in this experiment if any?
**Experiment 1: Does Enzyme Concentration affect Amylase Activity?** To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70 µl of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15-second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylose solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction. **Research Questions:** 1. Should enzyme concentration affect the time it takes the reaction to reach completion? Why? 2. We will investigate amylase concentrations of 1%, 0.5%, 0.25%, 0.125%. If you add equal amounts of enzyme to the same amount of substrate, which enzyme concentration do you think will break down the substrate first? Why? **Alternative Hypotheses:** - *H₀*: The speed of a reaction is not determined by the number of enzyme molecules available to catalyze it. The page does not include any graphs or diagrams.
Transcribed Image Text:**Experiment 1: Does Enzyme Concentration affect Amylase Activity?** To investigate the effect of enzyme concentration on amylase activity, we mixed 6 ml of 0.5% amylose solution with 70 µl of amylase enzyme solution. Each trial used a different concentration of enzyme solution. At 15-second intervals, we tested the reaction mixture for the presence of amylose starch by dropping the mixture into a well containing a drop of Lugol's iodine. We compared our results against the following: a well that contained only amylose solution and iodine, a well containing only enzyme solution and iodine, and a well containing completely digested (or broken-down) starch solution and iodine. We recorded the time of completion of the reaction for each of our trials, when no more starch was present, as the first sample that did not show a positive starch reaction. **Research Questions:** 1. Should enzyme concentration affect the time it takes the reaction to reach completion? Why? 2. We will investigate amylase concentrations of 1%, 0.5%, 0.25%, 0.125%. If you add equal amounts of enzyme to the same amount of substrate, which enzyme concentration do you think will break down the substrate first? Why? **Alternative Hypotheses:** - *H₀*: The speed of a reaction is not determined by the number of enzyme molecules available to catalyze it. The page does not include any graphs or diagrams.
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