The transport system of lactose in E.coli is shown below. Lactose transporter H+ H+ Lactose (outside) H+ H+ H* Proton pump H+ Lactose (inside) ADP +P, H* Ht ATP A) For each of the following statements determine if they are true (T) or false (F). The primary active transport of H' via proton pump, establishes a proton gradient across the membrane. I Select ) The intracellular pH is lower than the extracellular pH. [Select] • Lactose transporter is an example of antiport. [Select ] • Transport of lactose against its concentration gradient is entirely dependent on inflow of H down their electrochemical gradient. [Select) B) What would happen with the transport of lactose if an antimicrobial agent triclosan is added? Triclosan is a proton onophore. [Select] For the lactose transporter from the previous question, calculate the free-energy change for transporting 1 mol of protons into the cell? Membrane potential is -50 mv (inside negative) and the pH = 7.2 inside the cell and pH - 4.0 outside the cell. Temperature is 37"C (310 KL. Remember to convert pH to [H+] and express your answers as kJ/mol.
The transport system of lactose in E.coli is shown below. Lactose transporter H+ H+ Lactose (outside) H+ H+ H* Proton pump H+ Lactose (inside) ADP +P, H* Ht ATP A) For each of the following statements determine if they are true (T) or false (F). The primary active transport of H' via proton pump, establishes a proton gradient across the membrane. I Select ) The intracellular pH is lower than the extracellular pH. [Select] • Lactose transporter is an example of antiport. [Select ] • Transport of lactose against its concentration gradient is entirely dependent on inflow of H down their electrochemical gradient. [Select) B) What would happen with the transport of lactose if an antimicrobial agent triclosan is added? Triclosan is a proton onophore. [Select] For the lactose transporter from the previous question, calculate the free-energy change for transporting 1 mol of protons into the cell? Membrane potential is -50 mv (inside negative) and the pH = 7.2 inside the cell and pH - 4.0 outside the cell. Temperature is 37"C (310 KL. Remember to convert pH to [H+] and express your answers as kJ/mol.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Part A)
1. True
2. False
3. False
4. True
Part B)
Transport of lactose would increase.
![The transport system of lactose in E.coli is shown below.
Lactose
transporter
H+
H+
Lactose
(outside)
H+
H+
H*
Proton pump
H+
Lactose
(inside)
ADP +P,
H*
Ht
ATP
A) For each of the following statements determine if they are true (T) or false (F).
The primary active transport of H' via proton pump, establishes a proton gradient across the
membrane. I Select )
The intracellular pH is lower than the extracellular pH.
[Select]
• Lactose transporter is an example of antiport. [Select ]
• Transport of lactose against its concentration gradient is entirely dependent on inflow of H
down their electrochemical gradient. [Select)
B) What would happen with the transport of lactose if an antimicrobial agent triclosan is added?
Triclosan is a proton onophore.
[Select]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7270fe1e-0111-45ae-aa47-a2733f262922%2F32c1b78d-c574-4a9a-938a-27b139382e8a%2F05r299m_processed.png&w=3840&q=75)
Transcribed Image Text:The transport system of lactose in E.coli is shown below.
Lactose
transporter
H+
H+
Lactose
(outside)
H+
H+
H*
Proton pump
H+
Lactose
(inside)
ADP +P,
H*
Ht
ATP
A) For each of the following statements determine if they are true (T) or false (F).
The primary active transport of H' via proton pump, establishes a proton gradient across the
membrane. I Select )
The intracellular pH is lower than the extracellular pH.
[Select]
• Lactose transporter is an example of antiport. [Select ]
• Transport of lactose against its concentration gradient is entirely dependent on inflow of H
down their electrochemical gradient. [Select)
B) What would happen with the transport of lactose if an antimicrobial agent triclosan is added?
Triclosan is a proton onophore.
[Select]
![For the lactose transporter from the previous question, calculate the free-energy change for
transporting 1 mol of protons into the cell? Membrane potential is -50 mv (inside negative) and the
pH = 7.2 inside the cell and pH - 4.0 outside the cell. Temperature is 37"C (310 KL. Remember to
convert pH to [H+] and express your answers as kJ/mol.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7270fe1e-0111-45ae-aa47-a2733f262922%2F32c1b78d-c574-4a9a-938a-27b139382e8a%2F76dwsu6_processed.png&w=3840&q=75)
Transcribed Image Text:For the lactose transporter from the previous question, calculate the free-energy change for
transporting 1 mol of protons into the cell? Membrane potential is -50 mv (inside negative) and the
pH = 7.2 inside the cell and pH - 4.0 outside the cell. Temperature is 37"C (310 KL. Remember to
convert pH to [H+] and express your answers as kJ/mol.
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