To measure 15μl, which micropipette will you use? The windows in the micropipette will look like: To measure 60 μl, which micropipette will you use? The windows in the micropipette will look like: To measure 300 μl, which micropipette will you use? The windows in the micropipette will look like: To measure 4560 μl, which micropipette will you use? The windows in the micropipette will look like:
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- The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?How many coccus cells (0.0005 mm in diameter) could fit across the entire FOV of the 100x objective lens (assuming the DFOV of the 100x lens is 180 μm?What is the meaning of the different values given engraved on each objective?
- Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn vIf the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X objective, what is the true length of one cell in millimeters (mm)?Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Questions: What is the total magnification of the image under microscope? Show your solution. What is the distance of one ocular division? Show your solution. What is approximate cell length of Cell A, Cell B, and Cell C? What is approximate combine cell length of Cell A and Cell B? Show your solution. Remember: Always measure the cell length on the longest middle section of the cell!
- 3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTESa)What is the condenser lens on a compound microscope, and how can it affect your view of your specimen? b)Calculate the total magnification when looking through the 40X lens on the compound microscope. c) Describe the process to get a specimen into view using the 40X objective starting from the point after you have plugged in your scope and cleaned the objectives and slide.Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible
- The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?Two microscopes are available for students to use in your laboratory, with the following specifications: Table 1. Specifications of available microscopes in the laboratory. Microscope 1 Microscope 2 Eyepiece 10x 15x Objectives 4х, 10х, 40х, 100х 10х, 15х, 40х, 100х Numerical Aperture size 0.15, 0.25, 0.65, 1.4 0.25, 0.45, 0.65, 1.25 (per objective) A. Compute for the total magnification of an image under the LPO for both microscopes. B. Calculate the A and r for both microscopes given in Table 1. Find the actual size of the specimen or provide the appropriate conversions of the given sizes as needed to determine which of the two microscopes can you use for the following: 1. an image with 18x actual magnification and an apparent size of 1.04mm 2. unknown specimen that is 0.435Å 3. microorganism that is 2.1nm in size Table 2. Summary of computed values for two microscopes available in the laboratory. Microscope 1 Microscope 2 Total Magnification (LPO only) Wavelength of light (A) Limit…Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the distance of one ocular division? Show your solution.
