To measure 15μl, which micropipette will you use? The windows in the micropipette will look like: To measure 60 μl, which micropipette will you use? The windows in the micropipette will look like: To measure 300 μl, which micropipette will you use? The windows in the micropipette will look like: To measure 4560 μl, which micropipette will you use? The windows in the micropipette will look like:
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- The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?How many coccus cells (0.0005 mm in diameter) could fit across the entire FOV of the 100x objective lens (assuming the DFOV of the 100x lens is 180 μm?You are using a spectrophotometer to see whether you accurately performed a serial dilution of a colored water sample of unknown absorbance. You perform the following dilutions: Cuvet #1: 1600 uL of DI water + 400 uL of colored water Cuvet #2: 1600 uL of DI water + 400 uL of solution from Cuvet #1 Cuvet #3: 1600 uL of DI water + 400 uL of solution from Cuvet #2 What is the final dilution factor of your solution in Cuvet #3? 1:4 1:5 1:12 1:15 1:64 1:125 1:400
- What is the meaning of the different values given engraved on each objective?Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn vGiven the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700
- The BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.If the field diameter is 2.4 mm and there are 16 cells across the field of view when using the 40X objective, what is the true length of one cell in millimeters (mm)?Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Questions: What is the total magnification of the image under microscope? Show your solution. What is the distance of one ocular division? Show your solution. What is approximate cell length of Cell A, Cell B, and Cell C? What is approximate combine cell length of Cell A and Cell B? Show your solution. Remember: Always measure the cell length on the longest middle section of the cell!
- 3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTESa)What is the condenser lens on a compound microscope, and how can it affect your view of your specimen? b)Calculate the total magnification when looking through the 40X lens on the compound microscope. c) Describe the process to get a specimen into view using the 40X objective starting from the point after you have plugged in your scope and cleaned the objectives and slide.Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible