Three different Hfr donor strains are mixed with separate samples of an F strain, and the following mapping data are provided from studies of interrupted conjugation: Appearance of genes in F cells Hfr1: Genes b* d* c* f* g* Time* 3 16 27 59 Hfr2: Genes e* f* c* d* b+ Time 6 24 35 46 48 Hfr3: Genes d* c* f* e+ g* Time 4 15 26 44 58 Construct a genetic map for these genes, indicating their order on the bacterial chromosome and the distances between them.

Three different Hfr donor strains are mixed with separate samples of an F strain, and the following mapping data are provided from studies of interrupted conjugation: Appearance of genes in F cells Hfr1: Genes b* d* c* f* g* Time* 3 16 27 59 Hfr2: Genes e* f* c* d* b+ Time 6 24 35 46 48 Hfr3: Genes d* c* f* e+ g* Time 4 15 26 44 58 Construct a genetic map for these genes, indicating their order on the bacterial chromosome and the distances between them.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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3 2. The table below was obtained by P1 phage transduction followed by media selection of an E. coli strain lacking the three genes. Determine the map order of the genes given the information provided. Demonstrate CLEARLY which are closer or farther from each other (no numbers needed). 1 2 MAP: Experiment Selected marker Ala+ Gly+ Ser+ Unselected markers (select- ed for by subsequent plating) 27% Ser+, 2% Gly+ 3% Ser+, 1% Ala+ 35% Ala+, 4% Gly+
As mentioned in question 2 of More Genetic TIPS, origins oftransfer can be located in many different places on a bacterial chromosome,and their direction of transfer can be clockwise or counterclockwise.Let’s suppose a researcher conjugated six differentHfr strains that were thr + leu + tons str r azis lac + gal + pro + met +to an F − strain that was thr − leu − tonr str s azir lac − gal − pro −met −, and obtained the following results: Draw a map of the circular E. coli chromosome that shows thelocations and orientations of the origins of transfer in these six Hfrstrains.
A prototrophic strain (his" arg" lac') was used as a donor to transform an auxotrophic strain (his arg lac'). Initial transformants are isolated on minimal medium + histidine + arginine + lactose - glucose. i. What genotypes will grow on this medium? ii. These colonies are replicated to minimal medium + histidine, and 50% of the original colonies grow. What genotypes will grow on this medium? iii. The original colonies are also replicated to minimal medium + arginine, and 10% of the colonies grow. What genotypes will grow on this medium? iv. The original colonies are also replicated to minimal medium. No colonies grow. Based on this information, what genotypes will grow on minimal medium + histidine and on minimal medium + arginine? What is the relative gene order for his, arg, and lac? Which two genes are closer? Explain your answer.
The vector below was used in the cloning process. Xmnl 2294 (ii) Bcgl 2215 Scal 2177 (iii) Gsul 1784. Eco311 1766. Eam11051 1694, Cfr10l 1779. Eco01091 2674, Aatll 2617 Sspl 2501 bla (Ap) 2486 1626 146/ pUC18/19 2686 bp 1466 230 BstAPI 179 Ndel 183 Ehel 235 lacz 409 852 rep (pMB1) MCS Sapl 683 AWN 1217 Explain your results when observations as stated below were obtained after the plasmids were transformed into the host cells: (i) No colonies were growing on the LB plate added with ampicillin and X-gal. Afl, BspLU111 806 Only blue colonies were growing on the LB plate added with ampicillin and X-gal. Only white colonies were growing on the LB plate added with ampicillin and X-gal.
If a vector containing the desired gene X is inserted into a microorganism, for instance, A.carbornarious, and the protoplast transformation resulted in 9 gene X transformants, how to determine which transformant has the highest titer of gene X?
In 1944, Avery, Macleod, and McCarty provided strong evidence that DNA is the hereditary material in Streptococcus pneumoniae by Group of answer choices showing that avirulent cells could become virulent by the process of transduction none of these is true. showing that virulent cells could become avirulent if the DNA was destroyed after transformation showing that avirulent cells could not gain the ability to become virulent cells if conjugation was interrupted. showing that avirulent cells could not gain the ability to become virulent if DNA was destroyed after transformation.
7) The figures below depict nine interacting microbes. The center cell (black circle) is the altruist. Individuals that receive benefits from the altruist have white crosses. Dark blue individuals are clonemates. Individuals with small red circles are cooperators. Small green or yellow circles indicate different molecules on the surface of the cell. Which of is consistent with a greenbeard effect (phenotype matching)? the figures below a) A A d) D e) all of the above DO B В +4 ++ + D +
An interrupted mating study is carried out on Hfr strains 1, 2, and 3. After conjugation is established, a small sample of the mixture is collected every minute for 20 minutes to determine the distance between the genes on the chromosome. Times of entry for each of the three Hfr strains studied in the interrupted mating are provided in parenthesis. The total duration of conjugation (in minutes) is given for each transferred gene. Hfr Strain Genes Transferred Hfr 1 oriT(0) met(2) ala(8) lac(13) gal(17) Hfr 2 oriT(0) met(2) leu(7) thr(10) azi(17) Hfr 3 oriT (0) gal(3) pro(8) trp(14) azi(19) Hfr 4 oriT leu met ala lac Hfr 5 oriT trp azi thr leu a. Use the data to construct a circular map of the bacterium.
You co-culture the following bacterial strains: an Hfr prototroph and an F- auxotroph for the genes mal, met, mtl, and xyl. You interrupt conjugation at various time points and place the mixtures on media plates lacking each of the nutrients. Based on the results shown in the image, what is the order of these four genes along the bacterial chromosome? Please explain!
Explain the process of the DNA recombination for the production of Golden Rice from conventional white rice.
Compare and contrast conjugation involving an F+ donor, an Hfr strain, and an F' donor.
In a transformation experiment involving a wild type bacterial strain with a recipient strain with mutations in genes f, g, h and i pairs of genes were analyzed for co-transformation with the following results: Gene Pair Co-transformation g+ i+ f+ i+ yes no f+ h+ Уes f+ g+ no g+ h+ h+ i+ no уes What is the linear order of these genes relative to cach other?