These organic substrates have a [more/less/equivalent] negative free energy of hydrolysis than ATP. In glycolysis, 4 total ATP are produced in this way from a single molecule of glucose in [1/2/4] different enzymatic reactions/steps in the [investment/payoff] phase.

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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Substrate level phosphorylation is the spontaneous transfer of phosphate from an organic substrate to ADP. These organic substrates have a [more/less/equivalent] negative free energy of hydrolysis than ATP. In glycolysis, 4 total ATP are produced in this way from a single molecule of glucose in [1/2/4] different enzymatic reactions/steps in the [investment/payoff] phase.

 

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Introduction

Tasks performed by cells such as movement and synthesis of macromolecules requires energy. So most of the cell activity is dedicated to obtaining this free energy from the environment.  Adenosine triphosphate (ATP) is the cell energy currency. Cells obtain energy when the ATP is hydrolysed. 

Cell obtained this ATP from the glucose. The glucose is converted into energy form through a series of chemical reactions taking place in the cell. This reaction can be aerobic or anaerobic.

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