The energy for the production of ATP comes from the breakdown of a glucose molecule via many enzyme-mediated chemical reactions occurring at several distinct stages of cellular respiration. During this time, the chemical energy stored in glucose is continually trans- ferred across these reactions. Which of the following describes the form in which most of the energy from the glucose molecule is stored just before the oxidative phosphorylation stage in aerobic cellular respiration? A B с D acetyl CoA FADH₂ NADH pyruvate
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- iodoacetate is an irreversible inhibitor of glyceraldehyde-3-phosphate dehydrogenase. which of the following is the expected outcome of the exposure of a cell to iodoacetate - Cellular resiration will stop if the cell solely depends on glucose as fuel - The rate of cellular respiration will increase - The reoxidation of coenzymes will noy be affected , but the efficiency of oxidative phosphorylation will be diminished - There will be no effect on cellular respiration as long as the cell has a large glucose reserve -The cell sill not be able to reoxidize the reduced coenzymesIn glycolysis, the KM value of hexoquinase is 0.04 mM. At physiological conditions, the cellular concentration of glucose is between 4 mM to 15 mM. According with this information, which of the following statements is true (Choose all that are correct) The enzyme has a low affinity for its substrate The enzme is working at its maximum velocity The phosphorilation of glucose is very slow The small value of KM assures that glycolysis will continue even when the concentration of glucose drops below 4 mM. The hexoquinase is saturated with glucoseGlycolysis is the central metabolic pathway for any cell (prokaryotic or eukaryotic) to generate energy. The primary reactant that enter glycolysis is , and the process generates 2 (= reduced (= 3 carbon molecules.) co-enzyme, electron carrier molecule), and 2 Glucose (energy carrier molecule), 2 2 NAD+ 2 ADP 2NADH 2ATP Zw Pyruvate ●●● Pyruvate
- Stearic acid is an 18-carbon fatty acid. If a single molecular of stearic acid is within the cytosol of the cells: Describe the process by which stearic acid would be metabolised, beginning the molecule in the cytosol of the cell, ending with the creation of ATP, and assuming the cell has sufficient oxygen for all reactions to take place Showing all working, calculate how many ATP molecules could be generated from a single molecule of stearic acid in the cytosol of the cellConcerning the process of the aerobic fate of glucose oxidation, which of the following statements is false? O The spontaneous movement of protons is coupled to the unfavorable movement of electrons through the electron transport chain O The matrix of the mitochondria is more negative than the inner membrane space O Pyruvate is recognized bya pyruvate/proton symporter for movement into the matrix of the mitochondria O The movement of protons through ATP synthase drive the production of ATP O Mitochondria contain two membranes, only the inner mitochondrial membrane is highly selective for what passes across itBreak down the glycolysis pathway in terms of the following reaction steps (you may write or take photos of chemical reactions; be sure to include the enzyme involved): - ATP-requiring steps - ATP-producing steps - NAD-involving steps, and - Irreversible steps
- Concerning the process of the aerobic fate of glucose oxidation, which of the following statements is false? The spontaneous movement of protons is coupled to the unfavorable movement of electrons through the electron transport chain O The matrix of the mitochondria is more negative than the inner membrane space Pyruvate is recognized by a pyruvate/proton symporter for movement into the matrix of the mitochondria O The movement of protons through ATP synthase drive the production of ATP O Mitochondria contain two membranes, only the inner mitochondrial membrane is highly selective for what passes across itGive all the reactions that will produce ATP either by substrate-level phosphorylation (SLP) or by oxidative phosphorylation (OP). If the given require a shuttle system, please indicate both MA shuttle and GP shuttle and give the ATP produced. Given: fructose 6-phosphate to 2pyruvateConsider ten glucose molecules that enter a cell. How many ATP can be generated by the complete catabolism of these into CO2 and H2O? If all ten are first incorporated into glycogen, liberated from glycogen, and then fully catabolized into CO2 and H2O, does the ATP tally increase, decrease or stay the same? Consider that 1 UTP = 1 ATP. Explain. Describe the processes which produce ATP and provide a balanced equation of glucose, CO2, H2O and O2
- Assuming that the glycerol 3-phosphate shuttle is operative, a complete oxidation of five molecules of glucose (by a eukaryotic cell) into carbon dioxide and water would produce approximately _________ ATP molecules via oxidative phosphorylation only. (Consider NADH = 2.5 ATP and FADH2 = 1.5 ATP)Assuming that all the glucose entering a muscle fiber is oxidized, as what molecule will the glucose carbon leave the body? Of glucose’s six carbons, two oxidized carbon byproducts will be produced by one enzyme, and four will be produced by a metabolic pathway. At which enzyme and pathway are these carbon-based bi-products produced?Which of the following statements about the aerobic respiration of a molecule of glucose is FALSE? O It requires pathways and membrane complexes in both the cytosol and mitochondria in eukaryotes. O 34 molecules of ATP are generated by oxidative phosphorylation. O It requires pathways and membrane complexes in the cytosol and plasma membrane in prokaryotes. O It can generate 38 molecules of ATP in prokaryotes. O 12 molecules of ATP are generated by substrate-level phosphorylation.