The postgraduate student, Jocelyn, transformed her gene of interest into two different vectors; pUC18 and pZERO®-1. After that, she plated them on the antibiotic plates and incubated her samples for 18 hours. The next day, she realized that the plates were not labelled. Based on the observation above, can you identify which plates are carrying recombinant pUC18 and pZERO®- 1? Justify your answer.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
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