• The image shows the sequence of tandem repetitive DNA (highlighted in green) at onelocus in the genome, with the copy of the locus on each chromosome within thehomologous pair shown (and listed as #1 and #2). Answer the following questions.。 What type of repetitive DNA is shown? [Select]• You want to do a PCR of this locus. If one primer's sequence is 5'-TCGAGGTTC-3',then what is the second primer sequence needed to accurately PCR the locus?Choose a primer of 9 bases long.[Select]。 You follow the PCR with an agarose gel electrophoresis. When preparing for agarosegel electrophoresis, which electrode should be placed nearest the starting point ofthe sample (the product of the PCR)? [Select]。 Which allele would travel less far in your gel? [Select]#1:5'-TCGAGGTTCCAACAACAACAACAACAACAACAACAACAAGGATCTACT-3'3'-AGCTCCAAGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTCCTAGATGA-5'#2:5'-TCGAGGTTCCAACAACAACAACAACAACAACAACAACAACAACAACAACAAGGATCTACT-3'3'-AGCTCCAAGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTCCTAGATGA-5'

Type of repetitive DNA:The type of repetitive DNA shown is "microsatellite DNA." Microsatellites…

Answered: • The image shows the sequence of…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The following DNA fragment shows where a number of restriction endonucleases cut sites occur within the fragment. The numbers below indicate the distance between those cut sites in base pairs (bp). at of Koni Sall Eo R Pat Bam H Xhot Bem H Pat E Kon 100 200 300 400 s00 s00 700 00 00 1000 1100 1200 1300 1400 1600 1800 1700 1800 100 2000 The gel below shows: • a DNA ladder with markers every 200 bp (DNA Ladder) the resulting restriction fragments of the above DNA fragment if it was completely digested with the restriction endonuclease Xho I (Xho I Digest) the resulting restriction fragments of the above DNA fragment completely digested with unidentified restriction endonuclease(s) (lanes A through F) Which lane shows the DNA fragment completely digested with Pst ? Xho Digest Lanes DNA Ladder A. D F 2000 bp 1800 bp 1600 bp 1400 bp 1200 bp 1000 bp 800 bp 600 bp 400 bp 200 bp ||
19. You run a PCR of the above HBB sequence using the primers you designed. You receive a vector from a company that you want to clone your HBB PCR product into. Below is the vector: BamHI 0.5 kb EcoRI 1.0 kb 0.3 kb -Bglll plasmid A 0.2 kb BamHI 0.4 kb HindIII 1.0 kb EcoRI You want to cut this vector with BamHI and EcoRI and insert your PCR product into this vector. After cutting the vector with both enzymes simultaneously you run a gel. i) On the gel below draw the bands you expect to see after you cut this vector with these enzymes at the same time (only use one gel lane - the other one is going to be empty). (6 points) Ladder 5kb 3kb 2.5kb 2kb 1.5kb 1kb 0.5kb 0.3kb 0.2kb ii) Circle the band that represents the backbone that you will insert the PCR product into. (2 points) c) Once you have inserted the PCR product into the plasmid backbone from part C, how many bp will the new vector be (the total of the backbone and the PCR product together). Answer should be 2 significant figures.…
None
8. You perform a PCR experiment using the template and primers shown below. What size is the amplicon (aka the PCR product) in bp? Template Q8: 5' TCGGC CGTAC TACGA TCGAT TGCGT ACGCA ATCGC -3' ¦¦¦¦¦ ¦¦¦¦¦ ¦¦¦¦¦ ¦|||| |||||||||| 3' AGCCG GCATG ATGCT AGCTA ACGCA TGCGT TAGCG -5' Primers: 5'-CGTAC-3' & 5'-TTGCG-3'
An optimum ligation reaction should contain approximately 50 ng of vector. Given yourconcentrations recorded below, what is the volume of vector that should be added to eachligation reaction to have this mass of DNA in the reaction? Size of asPink-promoterless in bp: 702 (vector) Size of pCusC in bp: 157 The concentration of digested & purified PCR insert: 13.849 The concentration of digested and purified plasmid: 6.887 Any help with this is appreciated. I'm a bit confused thx
Design PCR primers for the DNA sequence given below and explain your choice. As part of your answer, draw a schematic of PCR primers binding to the DNA template. 5'-ATGGGGTCAGTCATGGTTA... .TTTATCGTGCATGTGATGTG-3'
5
The following chromatogram is obtained from the sequencing reaction. Using the key below, what message did you integrate into the yeast genome? AATATTAAAGTATAGAACGACTGTGAA A B с D E F G H I AAA AAT AAG AAC ATT ATG ATC AGT AGG J K L M N O P Q R Key GGG GGA GGT GGC GAA GAC GAT GTT GTA S T U V W X Y Z (SPACE) TTT TTC TTA TTG TGT TGA TGG TAA TAG
please answer all. I'll give thumbs up
Describe your amplicon based on molecular size. Comparing the size of the genomic DNA Describe your amplicon based on molecular size. Comparing the size of the genomic DNA (as seen in Fig. 8.1) and the PCR products based on band position in the gel (as seen in Fig. 8.2).
Answer the following: What is DNA fingerprinting? Name 2 applications of DNA fingerprinting from lecture. • Describe the steps of DNA fingerprinting. Include the following terms underlined: o DNA sample o PCR o restriction enzyme o gel electrophoresis If you haven't done so already, explain the steps of PCR in detail - also explain the steps of gel electrophoresis in detail.
Can you explain each of the statement given i didnt really understamd the dna recombinant

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