• The image shows the sequence of tandem repetitive DNA (highlighted in green) at onelocus in the genome, with the copy of the locus on each chromosome within thehomologous pair shown (and listed as #1 and #2). Answer the following questions.。 What type of repetitive DNA is shown? [Select]• You want to do a PCR of this locus. If one primer's sequence is 5'-TCGAGGTTC-3',then what is the second primer sequence needed to accurately PCR the locus?Choose a primer of 9 bases long.[Select]。 You follow the PCR with an agarose gel electrophoresis. When preparing for agarosegel electrophoresis, which electrode should be placed nearest the starting point ofthe sample (the product of the PCR)? [Select]。 Which allele would travel less far in your gel? [Select]#1:5'-TCGAGGTTCCAACAACAACAACAACAACAACAACAACAAGGATCTACT-3'3'-AGCTCCAAGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTCCTAGATGA-5'#2:5'-TCGAGGTTCCAACAACAACAACAACAACAACAACAACAACAACAACAACAAGGATCTACT-3'3'-AGCTCCAAGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTCCTAGATGA-5'

Type of repetitive DNA:The type of repetitive DNA shown is "microsatellite DNA." Microsatellites…

Answered: • The image shows the sequence of…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

2: Align DNA sequences using dot matrix Horizontal sequence: AGGCTCCC; vertical sequence: GCGCTCCG. Complete the dot matrix. Trace the features. Write the brief description of each feature below the matrix. For the dots, copy/paste this dot: • For noise reduction, use window size 2 x 2, mismatch limit 0, dot position 1, 1. Dot matrix after noise reduction: 3. Use the same sequences. For noise reduction, use window size 3 x 3, mismatch limit 1, dot position 3, 3. Dot matrix after noise reduction:
8. You perform a PCR experiment using the template and primers shown below. What size is the amplicon (aka the PCR product) in bp? Template Q8: 5' TCGGC CGTAC TACGA TCGAT TGCGT ACGCA ATCGC -3' ¦¦¦¦¦ ¦¦¦¦¦ ¦¦¦¦¦ ¦|||| |||||||||| 3' AGCCG GCATG ATGCT AGCTA ACGCA TGCGT TAGCG -5' Primers: 5'-CGTAC-3' & 5'-TTGCG-3'
Design PCR primers for the DNA sequence given below and explain your choice. As part of your answer, draw a schematic of PCR primers binding to the DNA template. 5'-ATGGGGTCAGTCATGGTTA... .TTTATCGTGCATGTGATGTG-3'
5
The following chromatogram is obtained from the sequencing reaction. Using the key below, what message did you integrate into the yeast genome? AATATTAAAGTATAGAACGACTGTGAA A B с D E F G H I AAA AAT AAG AAC ATT ATG ATC AGT AGG J K L M N O P Q R Key GGG GGA GGT GGC GAA GAC GAT GTT GTA S T U V W X Y Z (SPACE) TTT TTC TTA TTG TGT TGA TGG TAA TAG
please answer all. I'll give thumbs up
Describe your amplicon based on molecular size. Comparing the size of the genomic DNA Describe your amplicon based on molecular size. Comparing the size of the genomic DNA (as seen in Fig. 8.1) and the PCR products based on band position in the gel (as seen in Fig. 8.2).
Answer the following: What is DNA fingerprinting? Name 2 applications of DNA fingerprinting from lecture. • Describe the steps of DNA fingerprinting. Include the following terms underlined: o DNA sample o PCR o restriction enzyme o gel electrophoresis If you haven't done so already, explain the steps of PCR in detail - also explain the steps of gel electrophoresis in detail.
The results of the diagram (Agarose electrophoresis photodocumentation and quantification table) correspond to the information for 4 samples of genomic DNA extracted from plant tissue. With which sample (s) could a PCR be performed to amplify a 100 pb region? Al A2 АЗ А4 ID DNA 260 nm 260 nm (pb) 10,000 sample (ng/uL) 280 m 230 nm A1 170 1.9 2.0 А2 180 1.5 1.5 5,000 АЗ 170 1.7 1.5 А4 170 1.9 2.0 1,000 500 100 choose the best option: а. All b. None с. А2 d. A1, A2 е. А1
As the leading scientist in a biomedical science laboratory, it is a requirement to give advice to your lab assistants when they are having problems with their experiments. What advice would you give to your assistants that are having the following problems: After performing a polymerase chain reaction (PCR) and agarose gel electrophoresis to confirm the presence of the C01 gene of 750bp. 2.1. They observe no band appearing on an agarose gel. What would be your conclusion? 2.2. They observe three bands of different sizes that resemble a smear on the gel. Advice 2.3. They observe a single band on the gel and conclude that the PCR product is an exact copy of the original template DNA. Would you support their condusion? Explain. 2.4. Explain how PCR can be used to detect infectious agents in diagnoses of diseases.
Among all these primer pairs from Primer-Blast, which is the best to use for a PCR reaction and why?
Suppose that you want to sequence the following DNA fragment: 5′–TCCCGGGAAA-primer site–3′ You first use PCR to amplify the fragment, so that there is sufficient DNA for sequencing. You carry out dideoxy sequencing and then separate the products of the sequencing reactions by gel electrophoresis. Draw the bands that should appear on the gel from the four sequencing reactions.

Recommended textbooks for you

Human Anatomy & Physiology (11th Edition)

Biology

ISBN:

9780134580999

Author:

Elaine N. Marieb, Katja N. Hoehn

Publisher:

PEARSON

Biology 2e

Biology

ISBN:

9781947172517

Author:

Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:

OpenStax

Anatomy & Physiology

Biology

ISBN:

9781259398629

Author:

McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter

Publisher:

Mcgraw Hill Education,

Human Anatomy & Physiology (11th Edition)

Biology

ISBN:

9780134580999

Author:

Elaine N. Marieb, Katja N. Hoehn

Publisher:

PEARSON

Biology 2e

Biology

ISBN:

9781947172517

Author:

Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:

OpenStax

Anatomy & Physiology

Biology

ISBN:

9781259398629

Author:

McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter

Publisher:

Mcgraw Hill Education,

Molecular Biology of the Cell (Sixth Edition)

Biology

ISBN:

9780815344322

Author:

Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter

Publisher:

W. W. Norton & Company

Laboratory Manual For Human Anatomy & Physiology

Biology

ISBN:

9781260159363

Author:

Martin, Terry R., Prentice-craver, Cynthia

Publisher:

McGraw-Hill Publishing Co.

Inquiry Into Life (16th Edition)

Biology

ISBN:

9781260231700

Author:

Sylvia S. Mader, Michael Windelspecht

Publisher:

McGraw Hill Education

SEE MORE TEXTBOOKS