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The Hill coefficients for NAD+ binding to the T. tenax GAPDH in the presence and absence of NADP+ were measured and are shown in the graph in Task 16. What is the significance of the change in value of the Hill coefficient? Is this consistent with the shape of the curves on the graph (sigmoidal versus hyperbolic)? Explain. Is the change in value of the Hill coefficient consistent with the information given in the table in Task 16 about the enzyme’s structure - cooperativity implies the enzyme has subunits - do the molecular weight data concur?

The Hill coefficients for NAD+ binding to the T. tenax GAPDH in the presence and absence of NADP+ were measured and are shown in the graph in Task 16. What is the significance of the change in value of the Hill coefficient? Is this consistent with the shape of the curves on the graph (sigmoidal versus hyperbolic)? Explain. Is the change in value of the Hill coefficient consistent with the information given in the table in Task 16 about the enzyme’s structure - cooperativity implies the enzyme has subunits - do the molecular weight data concur?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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The Hill coefficients for NAD+ binding to the T. tenax GAPDH in the presence and absence of NADP+ were measured and are shown in the graph in Task 16. What is the significance of the change in value of the Hill coefficient? Is this consistent with the shape of the curves on the graph (sigmoidal versus hyperbolic)? Explain. Is the change in value of the Hill coefficient consistent with the information given in the table in Task 16 about the enzyme’s structure - cooperativity implies the enzyme has subunits - do the molecular weight data concur?
 
Units/mg protein 20 15 (TI Metabolite None NADP* -NADP- Glucose-1-phosphate AMP Hill coefficient = 2 10 [NAD], mM Apparent KM (mM) 3.0 Hill coeficient = 1 Control - AMP -X-Glucose-1-P 5.8 1.0 1.2 15 20 Activator or inhibitor? N/A Inhibitor activator. activator.
Transcribed Image Text:Units/mg protein 20 15 (TI Metabolite None NADP* -NADP- Glucose-1-phosphate AMP Hill coefficient = 2 10 [NAD], mM Apparent KM (mM) 3.0 Hill coeficient = 1 Control - AMP -X-Glucose-1-P 5.8 1.0 1.2 15 20 Activator or inhibitor? N/A Inhibitor activator. activator.
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