Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
The Hill coefficients for NAD+ binding to the T. tenax GAPDH in the presence and absence of NADP+ were measured and are shown in the graph in Task 16. What is the significance of the change in value of the Hill coefficient? Is this consistent with the shape of the curves on the graph (sigmoidal versus hyperbolic)? Explain. Is the change in value of the Hill coefficient consistent with the information given in the table in Task 16 about the enzyme’s structure - cooperativity implies the enzyme has subunits - do the molecular weight data concur?

The Hill coefficients for NAD+ binding to the T. tenax GAPDH in the presence and absence of NADP+ were measured and are shown in the graph in Task 16. What is the significance of the change in value of the Hill coefficient? Is this consistent with the shape of the curves on the graph (sigmoidal versus hyperbolic)? Explain. Is the change in value of the Hill coefficient consistent with the information given in the table in Task 16 about the enzyme’s structure - cooperativity implies the enzyme has subunits - do the molecular weight data concur?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
The Hill coefficients for NAD+ binding to the T. tenax GAPDH in the presence and absence of NADP+ were measured and are shown in the graph in Task 16. What is the significance of the change in value of the Hill coefficient? Is this consistent with the shape of the curves on the graph (sigmoidal versus hyperbolic)? Explain. Is the change in value of the Hill coefficient consistent with the information given in the table in Task 16 about the enzyme’s structure - cooperativity implies the enzyme has subunits - do the molecular weight data concur?
 
Units/mg protein 20 15 (TI Metabolite None NADP* -NADP- Glucose-1-phosphate AMP Hill coefficient = 2 10 [NAD], mM Apparent KM (mM) 3.0 Hill coeficient = 1 Control - AMP -X-Glucose-1-P 5.8 1.0 1.2 15 20 Activator or inhibitor? N/A Inhibitor activator. activator.
Transcribed Image Text:Units/mg protein 20 15 (TI Metabolite None NADP* -NADP- Glucose-1-phosphate AMP Hill coefficient = 2 10 [NAD], mM Apparent KM (mM) 3.0 Hill coeficient = 1 Control - AMP -X-Glucose-1-P 5.8 1.0 1.2 15 20 Activator or inhibitor? N/A Inhibitor activator. activator.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS