In an experiment, mitochondria were suspended in a buffered medium. The Oz electrode was used to monitor 0, consumption and assayed for the presence of ATP. Figure 3 shows the respiratory control by following the oxygen utilization (solid line) and ATP synthesis (discontinuous line) in the isolated mitochondria with additions of succinate, ADP, orthophosphate, oligomycin and dinitrophenol (DNP). Comment on the observations and relate the effects of the compounds added to electron transport and oxidative phosphorylation. Add DNP Add oligomycin Add ADP + P Add succinate Time Figure 3 Og consumed ATP synthosized

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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In an experiment, mitochondria were suspended in a buffered medium. The O2 electrode was
used to monitor 0, consumption and assayed for the presence of ATP. Figure 3 shows the
respiratory control by following the oxygen utilization (solid line) and ATP synthesis
(discontinuous line) in the isolated mitochondria with additions of succinate, ADP,
orthophosphate, oligomycin and dinitrophenol (DNP). Comment on the observations and relate the
effects of the compounds added to electron transport and oxidative phosphorylation.
Add
DNP
Add
oligomycin
Add
ADP + P
Add
succinate
Time
Figure 3
Og consumed
ATP synthesized
Transcribed Image Text:In an experiment, mitochondria were suspended in a buffered medium. The O2 electrode was used to monitor 0, consumption and assayed for the presence of ATP. Figure 3 shows the respiratory control by following the oxygen utilization (solid line) and ATP synthesis (discontinuous line) in the isolated mitochondria with additions of succinate, ADP, orthophosphate, oligomycin and dinitrophenol (DNP). Comment on the observations and relate the effects of the compounds added to electron transport and oxidative phosphorylation. Add DNP Add oligomycin Add ADP + P Add succinate Time Figure 3 Og consumed ATP synthesized
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