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The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Protein Molecular weight (Daltons) Isoelectric point Myoglobin 17,000 7.0 Cytochrome C 12,000 10.6 IgG 150,000 6.4 Hemoglobin 64,500 7.1 Insulin 5,800 5.4 Human serum albumin 66,000 4.6 Histone H3 15,000 10.8 Pepsin 33,000 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 2 4 pH 7 10 direction of electric current 150 MW (kDa) 100 В E 50 2 4 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein A В E F G H

The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Protein Molecular weight (Daltons) Isoelectric point Myoglobin 17,000 7.0 Cytochrome C 12,000 10.6 IgG 150,000 6.4 Hemoglobin 64,500 7.1 Insulin 5,800 5.4 Human serum albumin 66,000 4.6 Histone H3 15,000 10.8 Pepsin 33,000 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 2 4 pH 7 10 direction of electric current 150 MW (kDa) 100 В E 50 2 4 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein A В E F G H

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Molecular weight (Daltons) Protein Isoelectric point Myoglobin 17,000 7.0 Cytochrome C 12,000 10.6 IgG 150,000 6.4 Hemoglobin 64,500 7.1 Insulin 5,800 5.4 Human serum albumin 66,000 4.6 Histone H3 15,000 10.8 Рepsin 33,000 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 2 4 pH 7 10 direction of electric current 150 MW (kDa) 100 В E A 50 2 4 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein A D E
Transcribed Image Text:The following list of proteins are found in a mixture and are to be separated by 2D gel electrophoresis: Molecular weight (Daltons) Protein Isoelectric point Myoglobin 17,000 7.0 Cytochrome C 12,000 10.6 IgG 150,000 6.4 Hemoglobin 64,500 7.1 Insulin 5,800 5.4 Human serum albumin 66,000 4.6 Histone H3 15,000 10.8 Рepsin 33,000 1.0 In 2D electrophoresis, the mixture is first subjected to isoelectric focusing, separating them via their isoelectric pH values (corresponding to the horizontal axis, then subjected to SDS-PAGE to separate via molecular weight (corresponding to the vertical axis), where large proteins stay at top and smaller proteins go towards the bottom. 2 4 pH 7 10 direction of electric current 150 MW (kDa) 100 В E A 50 2 4 pH 7 10 With this information, match the letters of the bands to the proper protein based on the data given. Band Letter Protein A D E
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