The following table shows the distance moved in an SDS-polyacrylamide gel by a series of markerproteins of known relative molecular weight.a. If the tracking dye had moved a distance of 90.0 mm, calculate linear regression parameters such aslinearity coefficient (r), the slope (m), and the y-intercept (b).ProteinMW (DaDistance moved (mm)Transferrin78 0006.0Bovine serum albumin66 00012.5Ovalbumin45 00032.054.0TrypsinogenMyoglobinLysozymeCytochrome c24 00017 80069.014 30079.012 40086.5Proliferating cell nuclear antigen (PCNA) acts as a homotrimer of three identical subunits and helpsincrease the processivity of leading strand synthesis during DNA replication. It has a molecular weightof 36.0 kDa. Under the reducing conditions of the sample buffer, the disulfide bridges linking thesubunits are reduced and thus the monomer chains are seen on the gel.b. Calculate the Rf of the single band of PCNA when subjected to SDS-PAGE.c. Predict the distance (in mm) that will be traveled by the band. Report your answer in one (1) decimalplace.

The X-axis of the SDS PAGE Standard Plot is Relative Migration (also called Rf) , while the Y-axis…

Answered: The following table shows the distance…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You and Build Jakapan were tasked to determine the molecular weight of an unknown protein using gel filtration chromatography. You made use of a Sephadex G-100 column with a height of 30.0 cm and an internal volume of 52.0 mL. Initially, blue dextran was made to run through the column, giving an elution volume of 3.0 mL. Next, a calibration mixture containing phytochrome 730 (124 kDa), hemoglobin (64.5 kDa), and horse myoglobin (17 kDa) was made to flow into the column, generating a flow rate of 2.0 mL per minute. Shown as follows is the illustration of the collected fractions. Fraction number: ¯ÐÐ¯¯¤¤ÐÐ¶¶ÐÐ ÐÐ¡¡8 1 2 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Subsequently, a sample of the unknown protein was loaded into the column. The elution profile of the unknown protein is shown below. Absorbance at 254 nm 0.6 0.5 0.4 0.3 0.2 0.1 0 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Elution Volume, mL
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Marker Proteins Molecular Weight (daltons) Distance 132,000 66,000 43,000 14,400 Serum Albumin (Dimer) Serum Albumin (Monomer) 23mm 32mm Ovalbumin 39mm 60mm Lysozyme Unknown 1 26mm Unknown 2 58mm Rabbit serum (albumin) 33mm 1.On semilog paper (provided below) plot the distance migrated by each standard protein as a function of molecular weight. Determine the molecular weight of the unknown proteins and the major protein found in rabbit serum (albumin) from this calibration curve. ii) Interferon is a protein of 25,000 Dalton. How far would it have migrated, land it been included in this experiment? iii) The average amino acid residue in a protein has a molecular weight of 120 Daltons. Assuming that your unknowns are average, how many amino acid residues do they contain
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