The enzyme carbonic anhydrase can catalyze CO2 hydration according to the reaction below: CO2 + H2O The initial rates for the hydration reaction listed in the table to the right were obtained using an initial enzyme concentration of 2.3 nM and a temperature of 0.5 °C. Use a Lineweaver-Burk plot to find Km, the Michaelis constant, and k2, the turnover rate, for carbonic anhydrase in this reaction. HCO3 + H+ Rate (M s¹) [CO2] mM 2.78×10-5 5.00×10-5 1.25 2.5 8.33×10-5 1.67×10-4 5.0 20.0
The enzyme carbonic anhydrase can catalyze CO2 hydration according to the reaction below: CO2 + H2O The initial rates for the hydration reaction listed in the table to the right were obtained using an initial enzyme concentration of 2.3 nM and a temperature of 0.5 °C. Use a Lineweaver-Burk plot to find Km, the Michaelis constant, and k2, the turnover rate, for carbonic anhydrase in this reaction. HCO3 + H+ Rate (M s¹) [CO2] mM 2.78×10-5 5.00×10-5 1.25 2.5 8.33×10-5 1.67×10-4 5.0 20.0
Physical Chemistry
2nd Edition
ISBN:9781133958437
Author:Ball, David W. (david Warren), BAER, Tomas
Publisher:Ball, David W. (david Warren), BAER, Tomas
Chapter20: Kinetics
Section: Chapter Questions
Problem 20.38E: List at least four experimentally determined parameters that you, an experimenter, can define when...
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