TBS and TBST are used in the Western blots at different steps. 1. What is the difference between TBS and TBST and why is that extra “T” in TBST helpful for washing steps of the Western blot? (and ELISA…) Why does that extra “T” need to be removed before the detection step? For the transfer step of the Western Blot, why would we soak the membrane in transfer buffer before transfer? 2. If you forgot this step, how do you think your results would be affected?

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