TBS and TBST are used in the Western blots at different steps. 1. What is the difference between TBS and TBST and why is that extra “T” in TBST helpful for washing steps of the Western blot? (and ELISA…) Why does that extra “T” need to be removed before the detection step? For the transfer step of the Western Blot, why would we soak the membrane in transfer buffer before transfer? 2. If you forgot this step, how do you think your results would be affected?
TBS and TBST are used in the Western blots at different steps. 1. What is the difference between TBS and TBST and why is that extra “T” in TBST helpful for washing steps of the Western blot? (and ELISA…) Why does that extra “T” need to be removed before the detection step? For the transfer step of the Western Blot, why would we soak the membrane in transfer buffer before transfer? 2. If you forgot this step, how do you think your results would be affected?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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TBS and TBST are used in the Western blots at different steps.
1. What is the difference between TBS and TBST and why is that extra “T” in TBST helpful for washing steps of the Western blot? (and ELISA…) Why does that extra “T” need to be removed before the detection step?
For the transfer step of the Western Blot, why would we soak the membrane in transfer buffer before transfer?
2. If you forgot this step, how do you think your results would be affected?
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