Suppose a researcher conducts matings between three Hfr bacterial strains and an F¯ strain. The table shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Identify the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin. Hfr strain Order of gene transfer 1 ikvq w k t zr b m 3 v kimb i 2 1 m b Answer Bank
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Suppose a researcher conducts matings between three Hfr bacterial strains and an F− strain. The table shows the order in which genes are incorporated for each strain. Determine the gene order of the chromosome by placing each gene on the outside of the circular chromosome map. Identify the origin of replication for each Hfr strain on the inside of the map to indicate polarity of the origin.
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