Proteins at work at the replication fork - Topoisomerase Replication fork movement Helicase - Next Okazaki fragment ill start here. - RNA primer Primase - RNA primer Okazaki "fragment - Single-strand- binding proteins B clamp- DNA DNA Leading strand polymerase III dimer polymerase I Lagging strand - Ligase FIGURE 7-20 The replisome and accessory proteins carry out a number of steps at the repication fork. Topoisomerase and helicase unwind and open the double helix in preparation for DNA replication. When the double helix has been unwound, single-strand-binding proteins prevent the double helix from re-forming. The ilustration is a representation of the so-called trombone model (named for its resemblance to a trombone owing to the looping of the lagging strand) showing how the two catalytic cores of the replisome are envisioned to interact to coordinate the numerous events of leading- and lagging-strand replication. LaunchPad ANIMATED ART: Loading- and lagging-strand synthesis
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Referring to Figure 7-20, answer the following questions:
a. What is the DNA polymerase I enzyme doing?
b. What other proteins are required for the DNA
polymerase III on the left to continue synthesizing
DNA?
c. What other proteins are required for the DNA
polymerase III on the right to continue synthesizing
DNA?
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