A DNA strand was sequenced using the Sanger method(https://www.youtube.com/watch?v=KTstRrDTmWI). The reactiontube contained the DNA strand, fluorescently labelleddideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green,ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNApolymerase, or its Klenow fragment. Synthesis of DNA is allowed toproceed, and the results are shown on the right:151413121110(a) What is the sequence of the copy and the template strands?(b) If the template strand were in the 5'-3' direction, what will bethe sequence of the DNA copy?Nucleotide Length

A DNA primer complementary to the template DNA (the DNA to be sequenced) is used as a starting point…

Answered: a) What is the sequence of the copy and…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The -10 & -35 sequences specify __________ .Question 2 options: A) which strand is the template strand. B) which nucleotide is the start site of transcription. C) where an intron begins D) where mediator should bind E) both A and B are correct
A lagging strand is sketched below. The Okazaki fragment DNA is red, and the RNA primers are dashed orange lines. a) Which Okazaki fragment was made first, A, B, or C? b) When ligase joins fragments A and B, will it act at arrow 1, 2, or both?
DNA replication is semi-conservative, this statement means that Question 6 options: a) the new DNA molecules that are made are not identical to the original DNA molecule. b) the new DNA molecules that are made are only partially DNA since RNA primers are included. c) the new DNA molecules that are made are composed of one strand of the old DNA molecule and one strand of new DNA. d) of the two new DNA molecules made, one is entirely new DNA and one is entirely old new. e) the new DNA molecules that are made have a mixture of old and new DNA in each strand of the DNA duplex, randomly interspersed.
Name the enzymes that cut the DNA at specific sequences and create sticky ends.
What is the purpose of the dideoxynucleotide triphosphates in the Sanger sequencing reaction? A) The ddNTP prevents the denatured DNA in a sequencing reaction from re-forming a double helix. B The ddNTP terminates synthesis on a strand after it is incorporated by DNA polymerase. (C) The ddNTP acts as a catalyst for the DNA polymerase during in vitro DNA replication. The ddNTP is necessary to act as a primer in a sequencing reaction.
You are studying a colony of cells and determine that some of these cells have a mutated DNA polymerase I that results in loss of function of this enzyme. A) What will the effect of the mutation in DNA polymerase I be on DNA replication? In your answer make sure to describe what would be observed in the leading and lagging strand and explain your reasoning. B) Will this mutation in DNA polymerase I have an impact on another step in DNA replication? In your answer make sure to indicate whether DNA replication will be impacted or not. If it is not, explain why. If it is impacted, then describe the step that is impacted and name the molecule or enzyme involved.
1 10 5' АGT ССG A UGC 3' 8. There are inaccuracies in the DNA molecule shown here. || | | | | |||| 5 тCА GGCT АТG 3' a) Name three things that are wrong in the above DNA sequence. b) What type of chemical interaction is indicated by a "|" in the diagram? c) What happens to these interactions during DNA replication?
Is there any situation in which DNA is made based on a RNA template? What is the enzyme involved?
Replication slippage can cause indels in repetitive regions of DNA. Which of the following statements is not true?a) slippage of the new daughter strand causes repeat expansionb) slippage of the template strand causes repeat deletion c) slippage can cause indels on either the daughter or the template strandd) the indel mutation becomes fixed in one daughter cell's genome only after the next round of replication e) none of the above (all are true statements)
In E. Coli, which enzyme is responsible for removing and correcting mistake individual nucleotides during replication? O a) DNA Polymerase I b) RNA polymerase III O c) Nuclease d) DNA polymerase III
The leading and lagging strands of replicating DNA are growing in opposite directions; however, the extension of any DNA strand is always Question 10 options: a) 5' to 3'. b) 3' to 5'. c) either 5' to 3' or 3' to 5' depending on the direction of the replication fork. d) alternating between 5' to 3' and 3' to 5'. e) none of the above.
For the following short sequence of double stranded DNA and the given primers, there will be one major duplex DNA product after many cycles (imagine 10 cycles) of PCR. Provide the sequence of this one major duplex product and label the 5’ and 3’ ends of each strand. Sequence to be amplified: 5’- GGTATTGGCTACTTACTGGCATCG- 3’ 3’- CCATAACCGATGAATGACCGTAGC- 5’ Primers: 5’-TGGC-3’ and 5’-TGCC-3’

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a) What is the sequence of the copy and the template strands? b) If the template strand were in the 5'-3' direction, what will be the sequence of the DNA copy?