A DNA strand was sequenced using the Sanger method(https://www.youtube.com/watch?v=KTstRrDTmWI). The reactiontube contained the DNA strand, fluorescently labelleddideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green,ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNApolymerase, or its Klenow fragment. Synthesis of DNA is allowed toproceed, and the results are shown on the right:151413121110(a) What is the sequence of the copy and the template strands?(b) If the template strand were in the 5'-3' direction, what will bethe sequence of the DNA copy?Nucleotide Length

A DNA primer complementary to the template DNA (the DNA to be sequenced) is used as a starting point…

Answered: a) What is the sequence of the copy and…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Considering the Polymerase Chain Reaction (PCR), we can state that: a) It is a technique that promotes linear amplification of the number of DNA molecules in a sample. b) Depends on an initial template which can be a DNA or RNA molecule c) The reaction must contain, in addition to the template sequence, nitrogenous bases, sense and antisense primers and DNA polymerase enzyme. d)The primer sense rings on the template strand 3’>5’, extending from the 3’ end e)
a) If you isolated DNA from the ear and the tail of the same mouse, would you expect the DNA, isolated form the two tissue types, to be the same? Why? (2) b) Provide one difference between DNA replication in eukaryotes and prokaryotes with regards to their origin(s) of replication. (1) For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). 14px E EY A Y BIVS Paragraph Arial ABC x² X2 ?次T Te 田田国王用图 +]
The -10 & -35 sequences specify __________ .Question 2 options: A) which strand is the template strand. B) which nucleotide is the start site of transcription. C) where an intron begins D) where mediator should bind E) both A and B are correct
In which region(s) will Okazaki fragments be found during new strand synthesis? (see image for diagram) a) Region E only b) REgions E and F both c) Neither region E nor region F d) Region F only
b) For a DNA strand with the given genetic code of bases , undergoing transcription, what will be the complimentary RNA strand? Provide the direction as well as 5" and 3" indicators for the new genetic genome. 5" G-A-A-C-T-G-G-A^T-T-C-T-A-C-C3'.
a) How is the lagging strand made in DNA replication? Include important enzymes and structures. How is this different from the synthesis of the leading strand? How is the structure of mRNA suited to its function? How is the structure of tRNA suited to its function? How is the structure of the nucleus suited to its function for protein synthesis?
Telomerase is a reverse transcriptase enzyme that carries its own RNA molecule (Figure 1(a)). The telomerase enzyme attaches to the end of the telomeric region (Figure 1(b)) for DNA replication. (i) Indicate by drawing where the RNA of Telomerase binds to the telomeric region. W, X, Y, and Z are the ends of the DNA and RNA strands respectively. Identify ends of DNA's X, Y, and Z shown in Figure 1(a) & (b). (ii) (a) Telomerase -AAUCCCAAU- ITTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG-W' ПAАTСССААТСССААТСССАА-Х (b) Telomeric DNA Figure 1
In a double-stranded, B-form DNA, how many nucleotide bases can be found in 3 turns of the double helix?
A lagging strand is sketched below. The Okazaki fragment DNA is red, and the RNA primers are dashed orange lines. a) Which Okazaki fragment was made first, A, B, or C? b) When ligase joins fragments A and B, will it act at arrow 1, 2, or both?
DNA replication is semi-conservative, this statement means that Question 6 options: a) the new DNA molecules that are made are not identical to the original DNA molecule. b) the new DNA molecules that are made are only partially DNA since RNA primers are included. c) the new DNA molecules that are made are composed of one strand of the old DNA molecule and one strand of new DNA. d) of the two new DNA molecules made, one is entirely new DNA and one is entirely old new. e) the new DNA molecules that are made have a mixture of old and new DNA in each strand of the DNA duplex, randomly interspersed.
Name the enzymes that cut the DNA at specific sequences and create sticky ends.
What is the purpose of the dideoxynucleotide triphosphates in the Sanger sequencing reaction? A) The ddNTP prevents the denatured DNA in a sequencing reaction from re-forming a double helix. B The ddNTP terminates synthesis on a strand after it is incorporated by DNA polymerase. (C) The ddNTP acts as a catalyst for the DNA polymerase during in vitro DNA replication. The ddNTP is necessary to act as a primer in a sequencing reaction.

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a) What is the sequence of the copy and the template strands? b) If the template strand were in the 5'-3' direction, what will be the sequence of the DNA copy?