The figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box. Box A Box B Box C Box D Box E Box F DNA polymerase on lagging strand (just finishing an Okazaki fragment) F Maintains polymerase association with DNA Enzyme extends separation of DNA strands Synthesizes RNA fragments that hybridize to DNA Relaxes supercoiled DNA ahead of replication fork Maintains DNA is single stranded state Promotes binding of processivity factors to DNA A Newly synthesized strand booood Leading-strand template New Okazaki fragment RNA primer E Lagging-strand template DNA polymerase on leading strand B C D Saravastan - Next Okazaki fragment will start here Parental DNA helix

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## Eukaryotic Replication Machinery

The figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box:

- **Box A:** Maintains polymerase association with DNA
- **Box B:** Enzyme extends separation of DNA strands
- **Box C:** Synthesizes RNA fragments that hybridize to DNA
- **Box D:** Relaxes supercoiled DNA ahead of replication fork
- **Box E:** Maintains DNA in a single stranded state
- **Box F:** Promotes binding of processivity factors to DNA

### Detailed Diagram Explanation:

![Eukaryotic Replication Machinery](image-link)

The diagram provided illustrates the processes involved in eukaryotic DNA replication. It showcases various proteins and their interaction with DNA as they partake in the replication process. Here’s a detailed breakdown of each labeled part:

**Box A:** This represents a protein or complex that maintains the association of polymerase with DNA, ensuring that the DNA polymerase remains bound to the DNA strand during replication.

**Box B:** Here, an enzyme is illustrated that extends the separation of the DNA strands. This enzyme is crucial for unwinding the DNA double helix to allow replication to proceed.

**Box C:** This box signifies the enzyme responsible for synthesizing RNA primers. These RNA primers hybridize to the DNA to provide an initiation point for DNA synthesis.

**Box D:** This part shows a protein that relaxes supercoiled DNA ahead of the replication fork, preventing tangling and ensuring smooth progress of the replication machinery.

**Box E:** This protein maintains the DNA in a single-stranded state, preventing it from re-annealing so that replication can continue efficiently.

**Box F:** This area highlights a protein that promotes the binding of processivity factors to DNA, these factors are essential for high-speed DNA synthesis and stability of the replication machinery.

### Note:

Understanding these proteins and their functions provides significant insight into the complex yet meticulously coordinated process of eukaryotic DNA replication. Each component is critical for ensuring replication accuracy and efficiency, thus preserving genetic integrity during cell division.
Transcribed Image Text:## Eukaryotic Replication Machinery The figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box: - **Box A:** Maintains polymerase association with DNA - **Box B:** Enzyme extends separation of DNA strands - **Box C:** Synthesizes RNA fragments that hybridize to DNA - **Box D:** Relaxes supercoiled DNA ahead of replication fork - **Box E:** Maintains DNA in a single stranded state - **Box F:** Promotes binding of processivity factors to DNA ### Detailed Diagram Explanation: ![Eukaryotic Replication Machinery](image-link) The diagram provided illustrates the processes involved in eukaryotic DNA replication. It showcases various proteins and their interaction with DNA as they partake in the replication process. Here’s a detailed breakdown of each labeled part: **Box A:** This represents a protein or complex that maintains the association of polymerase with DNA, ensuring that the DNA polymerase remains bound to the DNA strand during replication. **Box B:** Here, an enzyme is illustrated that extends the separation of the DNA strands. This enzyme is crucial for unwinding the DNA double helix to allow replication to proceed. **Box C:** This box signifies the enzyme responsible for synthesizing RNA primers. These RNA primers hybridize to the DNA to provide an initiation point for DNA synthesis. **Box D:** This part shows a protein that relaxes supercoiled DNA ahead of the replication fork, preventing tangling and ensuring smooth progress of the replication machinery. **Box E:** This protein maintains the DNA in a single-stranded state, preventing it from re-annealing so that replication can continue efficiently. **Box F:** This area highlights a protein that promotes the binding of processivity factors to DNA, these factors are essential for high-speed DNA synthesis and stability of the replication machinery. ### Note: Understanding these proteins and their functions provides significant insight into the complex yet meticulously coordinated process of eukaryotic DNA replication. Each component is critical for ensuring replication accuracy and efficiency, thus preserving genetic integrity during cell division.
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