stGFP (-7) GFP(+36) GFP(-30)
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
Related questions
Question
You receive samples of the positively and negatively “supercharged”
green fluorescent proteins (GFP) developed by the Liu group at Harvard
(Lawrence et al. JACS 2007, 129, 10110.) along with standard GFP
(stGFP). In the picture below, blue represents a positive charge and red
negative charge on the surface of the proteins. Unfortunately the
technician that sent you the proteins forgot to label the tubes containing
the three different proteins. Explain how you would use chromatography
to identify which protein is which.
green fluorescent proteins (GFP) developed by the Liu group at Harvard
(Lawrence et al. JACS 2007, 129, 10110.) along with standard GFP
(stGFP). In the picture below, blue represents a positive charge and red
negative charge on the surface of the proteins. Unfortunately the
technician that sent you the proteins forgot to label the tubes containing
the three different proteins. Explain how you would use chromatography
to identify which protein is which.
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