The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.236. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express the answer in micromolar (notice micro means 106) Concentration of Blue Dye (M) Absorbance @ 630 nm 7.58x10-6 3.26x10-5 0.101 0.288 5.17x10-5 8.63x10-5 0.521 0.782

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
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The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the
following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown
concentration was 0.236. Use your graphing calculator to generate a standard curve from this data set, and
use it to determine the concentration of the unknown solution. Express the answer in micromolar (notice
micro means 10-6)
Concentration
|of Blue Dye (M)
Absorbance
@ 630 nm
7.58x10-6
0.101
3.26x10-5
5.17x10-5
8.63x10-5
0.288
0.521
0.782
Transcribed Image Text:The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.236. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express the answer in micromolar (notice micro means 10-6) Concentration |of Blue Dye (M) Absorbance @ 630 nm 7.58x10-6 0.101 3.26x10-5 5.17x10-5 8.63x10-5 0.288 0.521 0.782
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