1. The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows: [S] mol/l μmol/min 5.0 × 104 125 2.0 x 104 125 6.0 × 10-5 121 4.0 × 10-5 111 3.0 × 10-5 96.5 2.0 × 10-5 62.5 1.6 x 10-5 42.7 1.0 × 10-5 13.9 8.0 x 106 7.50 2. Describe how varying either Km and Vmax affects the rate of catalysis.
1. The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km by inspection. Plot the data using the Eadie-Hofstee method and determine these constants graphically. Explain the discrepancy in your two determinations. The initial rate data for the enzyme-catalyzed reaction are as follows: [S] mol/l μmol/min 5.0 × 104 125 2.0 x 104 125 6.0 × 10-5 121 4.0 × 10-5 111 3.0 × 10-5 96.5 2.0 × 10-5 62.5 1.6 x 10-5 42.7 1.0 × 10-5 13.9 8.0 x 106 7.50 2. Describe how varying either Km and Vmax affects the rate of catalysis.
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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![Chemical Engineering
1. The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km
by inspection. Plot the data using the Eadie-Hofstee method and determine these constants
graphically. Explain the discrepancy in your two determinations. The initial rate data for the
enzyme-catalyzed reaction are as follows:
[S]
mol/l
5.0 x 104
2.0 × 104
6.0 x 10-5
4.0 x 10-5
3.0 x 10-5
2.0 × 10-5
1.6 × 10-5
1.0 x 10-5
8.0 x 10-6
answer Q2 please
V
μmol/min
125
125
121
111
96.5
62.5
42.7
13.9
7.50
2. Describe how varying either Km and Vmax affects the rate of catalysis.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd7fa18b5-4d45-44d4-9e5b-ddce25eb0381%2F2ef6116b-fd78-487d-b803-27365368f945%2Fro3dpbe_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Chemical Engineering
1. The following data were obtained for an enzyme-catalyzed reaction. Determine Vmax and Km
by inspection. Plot the data using the Eadie-Hofstee method and determine these constants
graphically. Explain the discrepancy in your two determinations. The initial rate data for the
enzyme-catalyzed reaction are as follows:
[S]
mol/l
5.0 x 104
2.0 × 104
6.0 x 10-5
4.0 x 10-5
3.0 x 10-5
2.0 × 10-5
1.6 × 10-5
1.0 x 10-5
8.0 x 10-6
answer Q2 please
V
μmol/min
125
125
121
111
96.5
62.5
42.7
13.9
7.50
2. Describe how varying either Km and Vmax affects the rate of catalysis.
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